Neuroprotective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Oxygen-glucose Deprivation and Reperfusion in Cultured Hippocampal Cells
- VernacularTitle:人参皂甙Rg1对大鼠海马神经元缺糖氧/复糖氧后氧化损伤的保护作用
- Author:
Bin HE
;
Hong-hao WU
;
Jin-ru LÜ
;
Hao SUN
;
Hao WU
;
Lei JIANG
;
Gannan WANG
;
Deliang HU
;
Jinsong ZHANG
;
Yan CHEN
- Publication Type:Journal Article
- Keywords:
cerebral ischemia, Ginsenoside Rg1, glutathion, apoptosis, hippocampus
- From:
Chinese Journal of Rehabilitation Theory and Practice
2012;18(12):1112-1115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Ginsenoside Rgl on glutathion (GSH) level and glutathion peroxidase (GPx) activity after oxygen-glucose deprivation/reperfusion in cultured hippocampal cells. Methods The model of oxygen-glucose deprivation and reperfusion were established with the hippocampal neurons of rats. They were randomly divided into control group, model group and Ginsenoside Rgl treatment groups (5 μmol/L, 20 μmol/L, 60 μmol/L). The GSH level and GPx activity were measured 6 h after reperfusion. The apoptosis and the metabolic rate of methyl thiazolyl tetrazolium (MTT) were detected 24 h after reperfusion. Results Compared with model group, the GSH level, GPx activity, and metabolic rate of MTT improved (P<0.001), and the apoptosis decreased in the Ginsenoside Rgl groups (P<0.001) except with the dosage of 5 μmol/L (P>0.05). Conclusion Ginsenoside Rgl can protect the brain from ischemia by increasing the GSH level and GPx activity.
