Specific PCR for Identification of Astragalus membranaceus var. mongholicus Seeds， A. membranaceus Seeds， and Adulterants
10.13422/j.cnki.syfjx.20231211
- VernacularTitle:蒙古黄芪、膜荚黄芪及混伪品种子的位点特异性PCR鉴别
- Author:
Li LUO
1
;
Li HU
2
;
Chao JIANG
3
;
Ziyuan CHEN
3
;
Xiaolin LI
3
;
Yuan YUAN
1
Author Information
1. School of Traditional Chinese Medicine， Guangdong Pharmaceutical University， Guangzhou 510006， China
2. School of Pharmacy， Anhui University of Chinese Medicine， Hefei 230012， China
3. State Key Laboratory Breeding Base of Dao-Di Herbs， National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
- Publication Type:Journal Article
- Keywords:
Astragalus membranaceus var. mongholicus;
Astragalus membranaceus;
seed;
polymerase chain reaction
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(4):21-28
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a method based on specific polymerase chain reaction （PCR） that can accurately and rapidly identify Astragalus membranaceus var. mongholicus （AMM） seeds and A. membranaceus （AM） seeds. MethodThe Chloroplast Genome Information Resource （CGIR） and IdenDSS were used to obtain the characteristic DNA fragments of AMM and AM， and the specific single nucleotide polymorphism （SNP） sites of AMM and AM were screened out， on the basis of which the specific primers MG-F/MG-R of AMM and MJ-F/MJ-R of AM were designed. The specific PCR method for identifying AMM and AM was established and optimized， and the specificity and applicability of the method were investigated. ResultThe specific PCR conditions for the identification of AMM were primers MG-F/MG-R， annealing at 62 ℃， and 28 cycles. After PCR amplification and gel electrophoresis， the specific band appeared at about 220 bp， with no band for the seeds of AM or adulterants. The specific PCR conditions for identifying the AM were primers MJ-F/MJ-R， annealing at 58 ℃， and 28 cycles. After PCR amplification and gel electrophoresis， the band appeared at about 150 bp， with no band of AMM or adulterants. ConclusionThe specific PCR method established in this study can accurately and quickly identify the seeds of AMM and AM， which provides a basis for the classification and accurate identification of Astragalus seeds and adulterants.
