Enzyme-linked Immunosorbent Assay in Quality Control of Chinese Medicines： A Review
10.13422/j.cnki.syfjx.20231019
- VernacularTitle:酶联免疫吸附测定法用于中药质量控制的研究进展
- Author:
Yuansheng XU
1
;
Jiao LIU
2
;
Tian ZHANG
2
;
Yuyang ZHAO
2
;
Hui TIAN
1
;
Tiegui NAN
2
;
Yuan YUAN
2
Author Information
1. Guangxi University of Chinese Medicine， Nanning 530200， China
2. National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
- Publication Type:Journal Article
- Keywords:
quality control of Chinese medicine;
enzyme-linked immunosorbent assay;
toxic and harmful components;
pesticide residue;
active component
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(4):12-20
- CountryChina
- Language:Chinese
-
Abstract:
In the quality control of Chinese medicine， the detection of active components and toxic and harmful components are two important links. Although conventional methods such as high performance liquid chromatography and liquid chromatography-mass spectrometry can accurately quantify the above substances， they have shortcomings such as complicated operation， high costs， inability of detection at any time， difficult detection of insoluble and macromolecular substances. Enzyme-linked immunosorbent assay （ELISA） can adsorb antigens or antibodies on the surface of solid carriers and realize qualitative or quantitative analysis of targets by using the specific reactions of antigens and antibodies. This method is praised for the simple operation， high sensitivity， strong specificity， simple requirements for experimental equipment， a wide application range， and low costs. In recent years， ELISA has been widely used in the quality control of Chinese medicine， especially in the content determination of mycotoxins represented by aflatoxin and the qualitative and quantitative analysis of active components. ELISA plays an increasingly important role with its unique advantages， providing new methods and ideas for the rapid quality examination of large quantities of Chinese medicines. This paper reviews the research progress in ELISA for the quality control of Chinese medicine in recent years and prospects its technical development and application prospects， aiming to provide reference and research ideas for further using this method to ensure the quality， safety， and controllability of Chinese medicine.
