Effect of ion-exchange chromatography on removal of fibronectin in von Willebrand factor
10.13303/j.cjbt.issn.1004-549x.2023.03.019
- VernacularTitle:离子交换层析法对人血管性血友病因子中人纤维连接蛋白去除效果的研究
- Author:
Zheng ZHOU
1
;
Xuecheng ZHANG
1
;
Minghua WANG
1
;
Jiawen LI
1
;
Xiaowei MA
1
Author Information
1. Hualan Biological Engineering, Inc., Xinxiang 453000, China
- Publication Type:Journal Article
- Keywords:
von willebrand factor;
ion-exchange chromatography;
human fibronectin;
vWF titer recovery;
Fn removal rate
- From:
Chinese Journal of Blood Transfusion
2023;36(3):266-268
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To study the removal effect of fibronectin(Fn) from von willebrand factor(vWF) by ion-exchange chromatography through processing human coagulation factor Ⅷ chromatographic washing products, in order to select a method that can effectively reduce Fn without compromising the activity yield. 【Methods】 In a multi-batch process development experiment, Fractogel® EMD TMAE(M) strong anion filler produced by Merck(Germany) was used to conduct chromatography to investigate vWF ristomycins titer (vWF: RCof), vWF recovery, protein content and Fn content. 【Results】 During the development of vWF pilot purification process, the content of Fn in the samples can be effectively reduced by ion-exchange chromatography, with removal rate more than 87%, titer recovery of vWF more than 80%, and no significant change in other quality indexes. 【Conclusion】 The use of ion-exchange chromatography to purify vWF can effectively reduce the content of Fn, which has positive significance for developing new product process and improving the product quality of blood products manufacturers.
