Mitigating of the interference of anti-CD47 monoclonal antibody on transfusion compatibility detection
10.13303/j.cjbt.issn.1004-549x.2023.03.011
- VernacularTitle:抗-CD47单抗对输血相容性检测的干扰及处理
- Author:
Yiyang LYU
1
;
Wenbing KONG
1
;
Xiaogang CHEN
1
;
Chixiang LIU
1
;
Piao LYU
1
;
Hui ZHAO
1
;
Xue LIN
1
;
Huayou ZHOU
1
Author Information
1. Department of Blood Transfusion Medicine, South Hospital, South Medical University, Guangzhou 510515, China
- Publication Type:Journal Article
- Keywords:
CD47 monoclonal antibody;
pre-transfusion compatibility detection;
interference;
transfusion efficacy;
evaluation
- From:
Chinese Journal of Blood Transfusion
2023;36(3):238-241
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To evaluate the interference of anti-CD47 monoclonal antibody on transfusion compatibility detection, in order to establish methods for removing interference and evaluate its efficacy. 【Methods】 Blood samples from 8 patients in our clinical trial who were treated with anti-CD47 monoclonal antibody from Tianjing and Xinda were collected. ABO and Rh blood group antigen identification, direct anti-human globulin test, unexpected antibody screening test and cross-matching test were performed by ZZAP, Gamma-clone(an anti-globulin reagent lacking IgG4) and Immucor Capture-R solid phase agglutination kit. 【Results】 ABO blood group identification of 5 subjects were interfered after treatment with anti-CD47 monoclonal antibody. All 8 subjects showed 2+ to 4+ agglutination intensity on direct anti-human globulin test and 3+ to 4+ on unexpected antibody screening. The results of unexpected antibody screening by Gamma-clone and Immucor Capture-R solid phase agglutination kit were all negative, while the cross-matching test were compatible. Patients with anemia caused by CD47 monoclonal antibody treatment were transfused with 2 U suspension red blood cells, and the evaluation showed that the transfusion was effective. 【Conclusion】 The CD47 monoclonal antibody can interfere with transfusion compatibility detection, and the use of antiglobulin reagents lacking IgG4 and Immucor Capture-R solid phase agglutination kit can remove the interference, with good transfusion efficacy in patients.
