Comparison of yield of microorganisms in cord blood between two automated blood culture systems
10.13303/j.cjbt.issn.1004-549x.2023.03.004
- VernacularTitle:两种全自动血培养系统的脐带血微生物检出率比较
- Author:
Hui CAO
1
;
Xingjie LI
1
;
Yang CHEN
1
;
Qian CHEN
1
;
Jun HUANG
1
;
Qiuxia LIAO
1
;
Qiang CHEN
1
,
2
Author Information
1. Sichuan Cord Blood Bank, Sichuan Neo-life Stem Cell Biotech Inc, Chengdu 610036, China
2. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College
- Publication Type:Journal Article
- Keywords:
cord blood stem cells;
microbial contamination;
blood culture
- From:
Chinese Journal of Blood Transfusion
2023;36(3):211-214
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To compare the difference in the detection rate of microorganisms in cord blood between BACTEC FX and BacT/ALERT 3D automated blood culture systems, and to compare the influence of incubation time and different types of culture sample on the detection rate of microorganisms in cord blood. 【Methods】 Cord blood samples prepared from April to August 2020 in Sichuan Cord Blood Bank(n=4 358) were selected, and 20 mL of plasma was used as culture samples for microbial detection. In addition, cord blood samples prepared in the same months of 2021(n=4 057) were selected, and 19 mL of plasma plus 1 mL of final product was used as culture samples for microbial detection. The total sample size was 8 415, of which 4 849 samples(2 458 in plasma group and 2 391 in plasma plus final product group) were assigned to the BACTEC FX system, and 3 566 samples(1 900 in the plasma group and 1 666 in the plasma plus final product group) to the BacT/ALERT 3D system. All samples were cultured for 7 days, and culture data were recorded on day 5 and day 7. Positive results were confirmed by Gram staining. 【Results】 The positive rate detected by the BACTEC FX system was higher than that of the BacT/ALERT 3D system(4.08% vs 2.69%), with statistically significant difference(P<0.05). The positive rates of BACTEC FX and BacT / ALERT 3D on day 7 increased by 8% and 4.26% respectively compared with on day 5. When the culture sample types were concerned, the BACTEC FX showed statistically significant difference in positive rate between plasma group and plasma plus final product group(P<0.05), while no significant difference was found in positive rate(P>0.05) detected by the BacT/ALERT 3D system. With quality control strains, there were significant differences in TTP between these two systems for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Clostridium sporogenes, and Bacillus subtilis(P<0.05), but no significant difference for Aspergillus brasiliensis and Candida albicans(P>0.05). 【Conclusion】 This study suggests that the selection of BACTEC FX blood culture system with incubation time of not less than 7 days and plasma plus final product as culture samples may improve the detection rate of microorganisms in cord blood.
