Establishment of platelet donor gene bank involving specific antigen HPA 1-6/10/15/21 by real-time fluorescent PCR
10.13303/j.cjbt.issn.1004-549x.2023.03.002
- VernacularTitle:应用实时荧光PCR法建立献血者血小板特异性抗原HPA-1～6/10/15/21基因分型资料库
- Author:
Wensheng YUAN
1
;
Qianying CHEN
1
;
Huiyan LIN
1
;
Yuru FANG
1
;
Xiaolan ZHENG
1
;
Yongqing KUANG
1
Author Information
1. Zhongshan Central Blood Station, Zhongshan 528400, China
- Publication Type:Journal Article
- Keywords:
real time fluorescent PCR;
platelet specific antigen;
gene frequency
- From:
Chinese Journal of Blood Transfusion
2023;36(3):204-208
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To research the genetic polymorphism of HPA 1-6/10/15/21 in platelet donors in Zhongshan area, and establish a gene bank of platelet donors with HPA locus. 【Methods】 The HPA 1-6/10/15/21 system genotyping was performed by Real time fluorescent PCR combined with TaqMan probe technology on 192 platelet donors in Zhongshan area, and the genotype frequency and gene frequency were calculated. 【Results】 Only HPA-aa genotype was found within HPA-4/10, and no allele HPA-b had been detected. The majority of HPA-1, 2, 5, 6 and 21 genotypes were aa. HPA-3 and HPA-15 showed high heterozygosity, with genotype frequency of 0.307 3, 0.494 8 and 0.197 9 for HPA- 3aa, HPA-3ab and HPA-3bb, while 0.270 8, 0.505 2 and, 0.224 0 for HPA -15aa, HPA-15ab and HPA-15bb, respectively. 【Conclusion】 The distribution characteristics of HPA 1-6 /10/15/21 of platelet donors in Zhongshan shows regional differences compared with similar researches from other regions. The establishment of HPA gene bank is helpful to avoid alloimmunization caused by incompatible platelet transfusion.
