Characteristics analysis of HBV serological markers of NAT reactive blood donors under different HBsAg status
10.13303/j.cjbt.issn.1004-549x.2023.01.017
- VernacularTitle:HBsAg不同状态下NAT反应性献血者血清学标志物特征分析
- Author:
Dequn NI
1
;
Tingting WANG
1
;
Ting WANG
1
;
Mingrui LI
1
;
Weiya CUI
1
;
Xin XIE
1
;
Qing HE
1
;
Suping LI
1
Author Information
1. Anhui Blood Center, Hefei 230031, China
- Publication Type:Journal Article
- Keywords:
NAT;
HBsAg;
HBV;
Hepatitis B virus;
serological markers
- From:
Chinese Journal of Blood Transfusion
2023;36(1):69-72
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the characteristics of HBV serological markers of NAT reactive blood donors under different HBsAg status. 【Methods】 NAT reactive samples, with HBsAg-, HBsAg+ /retest - and HBsAg+ by single reagent were collected from September 2021 to May 2022 in our laboratory. The TMA non-reactive samples were retested by Roche PCR, then HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc were detected by ECLI for statistical analysis. 【Results】 A total of 66 samples were collected, among which 55 were HBsAg-/NAT+. The positive rate of anti-HBc, anti-HBs+ anti-HBc, anti-HBe+ anti-HBc was 87.3% (48/55), 43.6% (24/55) and 45.5% (25/55), respectively. The positive rate of anti-HBs was 10.9% (6/55) and the overall negative rate was 1.8% (1/55). In 7 HBsAg+ initially/retest -/NAT+ samples, the positive rate of anti-HBc was 100%(7/7), and the positive rate of anti-HBe+ anti-HBc was 71.4%(5/7). In 4 HBsAg+ /NAT+ samples by single reagent, the positive rate of HBsAg+ anti-HBs+ anti-HBe+ anti-HBc was 50% (2/4), and positive rate of anti-HBe+ anti-HBc was100% (4/4). Samples, not reactive to TMA discriminatory and anti-HBc negative, were also non-reactive to individual PCR retest. There were significant differences in the positive rates of anti-HBe+ anti-HBc between HBsAg-/NAT+ samples and HBsAg+ /NAT+ (single reagent) samples (P<0.05). 【Conclusion】 Most HBsAg-/NAT+ blood donors were occult hepatitis B virus infection.The anti-HBe+ anti-HBc positive were correlated with HBV infection status. Non-reactivity discriminated by TMA plus anti-HBc negative do not exclude HBV DNA non-reactivity.
