Protective effect of platelet-rich plasma on LPS-induced neuroinflammation in BV2 microglia through NRF2/HO-1 pathway
10.13303/j.cjbt.issn.1004-549x.2023.01.005
- VernacularTitle:PRP通过NRF2/HO-1通路对LPS诱导的BV2小胶质细胞神经炎症的保护作用
- Author:
Yinghui WANG
1
;
Ya ZHANG
2
;
Zilin WANG
2
;
Zixin ZHU
3
;
Qiuju MOU
3
;
Lili ZHU
2
,
4
Author Information
1. Department of Medical Laboratory, The People′s Hospital of Qiannan, Duyun 558000, China
2. School of Medical Laboratory Science, Guizhou Medical University
3. Department of Blood Transfusion, The Affiliated Hospital of Guizhou Medical University
4. Department of Blood Transfusion, The Affiliated Hospital of Guizhou Medical University
- Publication Type:Journal Article
- Keywords:
platelet-rich plasma;
BV2 microglia;
neuroinflammation;
oxidative stress;
NRF2/HO-1 signaling pathway
- From:
Chinese Journal of Blood Transfusion
2023;36(1):19-25
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the protective effect and mechanism of platelet-rich plasma (PRP) on lipopolysaccharide (LPS) -induced inflammatory response in BV2 cells. 【Methods】 BV2 microglia were divided into normal control group, 10%PRP control group, LPS group (LPS induction), 3%PRP+ LPS group (LPS induction, 3%PRP pretreatment), 5%PRP+ LPS group (LPS induction, 5%PRP pretreatment), 10%PRP+ LPS group (LPS induction, 10%PRP pretreatment), and the proliferation of BV2 cells was measured by CCK-8. The mitochondrial membrane potential of BV2 cells was measured by confocal microscopy, ROS was measured by fluorescence method, and NO was measured by Griess method. The protein expressions of IL-6, TNF-α, BACH1, GPX4, NRF2 and HO-1 were detected by Western blot. In addition, BV2 microglia were treated with HO-1 inhibitor and divided into normal control group, LPS group, ZnPP+ LPS group, 10%PRP+ LPS group, ZnPP+ LPS+ 10%PRP group, and the protein expressions of HO-1, IL-6 and TNF-α were detected by Western blot. 【Results】 Compared with normal control group, PRP promoted the proliferation of BV2 cells (P<0.01). The mitochondrial membrane potential decreased, ROS production increased, the levels of NO, IL-6, TNF-α and BACH1 increased (P<0.01). However, the expression levels of GPX4, NRF2 and HO-1 decreased (P<0.01) in LPS group. Compared with LPS group, the proliferation activity and mitochondrial membrane potential of BV2 cells in 3%PRP+ LPS, 5%PRP+ LPS and 10%PRP+ LPS groups significantly increased. The levels of ROS, NO, IL-6, TNF-α and BACH1 significantly decreased (P<0.01). The expressions of GPX4, NRF2 and HO-1 in different concentrations of PRP (3%, 5% and 10%) increased (P<0.01). Moreover, the expression of IL-6 and TNF-α in ZnPP+ LPS group was significantly higher than that in LPS group after HO-1 inhibitor treatment. Compared with 10%PRP+ LPS+ ZnPP group, HO-1 inhibitor could reverse the effect of PRP on the expression of IL-6 and TNF-α in LPS-induced BV2 cells (P<0.01). 【Conclusion】 PRP inhibits the inflammatory response of BV2 microglia induced by LPS by activating the NRF2/HO-1 signaling pathway.
