Genotyping identification and sequence analysis of ABO ambiguous blood group in 20 cases
10.13303/j.cjbt.issn.1004-549x.2023.01.002
- VernacularTitle:ABO疑难血型的基因型鉴定与序列分析
- Author:
Qiuyan LIN
1
;
Jinping ZHANG
2
;
Zhenyu HUANG
3
;
Qinghua HUANG
1
;
Liping FAN
1
;
Danhui FU
1
;
Haobo HUANG
1
Author Information
1. Department of Blood Transfusion, Fujian Medical University Union Hospital, Fuzhou 350001, China
2. Department of Blood Transfusion, Fuzhou Second Hospital
3. School of Food and Bioengineering, Fujian Polytechnic Normal University
- Publication Type:Journal Article
- Keywords:
ABO blood group;
ambiguous blood group;
genotype;
sequence analysis
- From:
Chinese Journal of Blood Transfusion
2023;36(1):8-10
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To study the genotypes of ABO ambiguous blood group samples(n=20) and identify their molecular biological characteristics. 【Methods】 The serological phenotype of the samples was analyzed by serological techniques. Seven exons of ABO gene were amplified by polymerase chain reaction (PCR) and the PCR products were directly sequenced; the genotypes and sequences of ABO subtypes were analyzed. 【Results】 The serological phenotypes of 20 samples presenting ABO ambiguous blood group were as follows: weak A antigen (n=5), weak A antigen combined with anti-A1 antibody (n=5), normal A antigen combined with anti-A1 antibody (n=2), weak B antigen (n=8). The genotypes of them were as follows: Ax02/O01 (n=3), Ael07/O01 (n=2), B313/O01 (n=2), A204/O02 (n=1), A220/O01 (n=1), Ael07/O02 (n=1), Ael02/O01 (n=1), Ael02/O02 (n=1), Ax03/O01 (n=1), Ax03/O02 (n=1), B313/O02 (n=1), B302/O01 (n=1), B302/O02 (n=1), Bw19/O02 (n=1), A102/B313 (n=1) and A101/Bw37 (n=1). 【Conclusion】 ABO genotyping technology can accurately identify the ambiguous blood group of samples, provide definite genetic information of blood group and ensure the safety of clinical transfusion.
