Detection results of blood donors with HBsAg reactivity to single reagent: a retrospective analysis
10.13303/j.cjbt.issn.1004-549x.2023.02.017
- VernacularTitle:HBsAg单试剂反应性献血者检测结果追溯性分析
- Author:
Rui HU
1
;
Tingting WANG
1
;
Qing LYU
1
;
Panpan WANG
1
;
Mingrui LI
1
;
Ting WANG
1
Author Information
1. Anhui Blood Center, Hefei 230031, China
- Publication Type:Journal Article
- Keywords:
HBsAg;
sole reagent reactive;
blood donors;
electrochemiluminescence immunoassay (ECLIA)
- From:
Chinese Journal of Blood Transfusion
2023;36(2):164-167
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To retrospectively analyze the detection results of blood donors with HBsAg reactivity to single reagent detected by enzyme-linked immunosorbent assay (ELISA) in our center, so as to provide basis for further consolidating the blood donor team. 【Methods】 Samples of blood donors who had been deferred for at least 6 months due to HBsAg reactivity to sole ELISA assay were collected, and HBsAg ELISA and NAT were further performed. Meanwhile, HBsAg/HBsAb/HBeAg/HBeAb/HBcAb were detected by Roche electrochemiluminescence immunoassay, and the results were statistically analyzed. 【Results】 Among these 51 selected samples, 45 were negative to two assays, 6 were reactive to sole assay, with reactivity-yield rate at 11.76% (6/51). The results of NAT/ECLIA were all negative. For five indicators of hepatitis B virus infection, 23 samples were all negative and 28 were partially positive, mainly anti-HBs, anti-HBc and anti-HBe. 【Conclusion】 The follow-up detection of HBsAg ELISA sole-reagent reactive samples, supplemented with the detection of HBV serological markers, can reduce the number of deferred blood donors, increase the willingness to donate blood again, and protect the rights and interests of blood donors.
