Preparation and preliminary evaluation of sodium alginate/agarose/carboxymethyl chitosan spray film dressing carrying platelet rich plasma for wound repair
10.13303/j.cjbt.issn.1004-549x.2023.02.010
- VernacularTitle:载富血小板血浆海藻酸钠/琼脂/羧甲基壳聚糖创面修复喷膜敷料的研制及初步性能评价
- Author:
Yubing XU
1
;
Zhanhong ZHU
1
;
Guangya LIU
1
;
Li CHEN
1
;
Zijing ZHU
1
;
Mou ZHOU
1
;
Guiqiu SHAN
1
Author Information
1. Department of Blood Transfusion Medicine, General Hospital of Southern Theater Command, Guangzhou 510010, China
- Publication Type:Journal Article
- Keywords:
platelet rich plasma;
sodium alginate/agarose/carboxymethyl chitosan;
spraying film;
wound repair
- From:
Chinese Journal of Blood Transfusion
2023;36(2):136-140
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To develop a spray-on membrane dressing for wound repair containing platelet rich plasma (PRP) sodium alginate (SA)/agarose(AG)/carboxymethyl chitosan (CMCS). 【Methods】 SA/AG/ CMCS were mixed in different proportions to prepare biodegradable quick setting spray (BQSS) by blending film method, and the film-forming time, moisture retention and compression resistance of the prepared BQSS were tested. Then PRP and BQSS were mixed in the proportion of 3∶7, 4∶6, 5∶5, 6∶4 and 7∶3 to prepare PRP-BQSS spray film dressings. The film-forming time, moisture retention, compressive strength, porosity and slow-release effect of growth factors of PRP-BQSS spray film dressings were studied. 【Results】 In the preparation of BQSS compound spray film solution, when SA, AG, CMCS and sterile distilled water were 0.6∶0.6∶0.6∶98.2g, the film-forming time (7.73±0.31) s, moisture retention (75. 54±3.03) % and compression resistance (791.00±68.02) g of the spray-film dressing were the best. The basic properties of PRP-BQSS spray-on film dressings and the release of growth factors show that PRP-BQSS spray-on film dressings can exist in different forms, and with the decrease of PRP concentration percentage, its film-forming time, moisturizing performance and compressive strength showed an upward trend. When the PRP content is 30%, the porosity of the dressing is the highest, about(84.34±0.90)%. The release of platelet-derived growth factor-AA(PDGF-AA), platelet factor-4(PF-4) and transforming growth factor beta (TGF-β) was in a slow upward trend, and the release of the three growth factors was higher than that of PRP group in 48 hours. 【Conclusion】 The preparation method of PRP-BQSS spray film dressing designed in this study is simple and mild, and can form a film quickly, with good biological properties and better growth factor inhibition and sustained-release effect.
