Effect of IgG dimer concentration on the binding ability of IgG Fc fragment in IVIG to THP-1 cell surface receptors
10.13303/j.cjbt.issn.1004-549x.2023.02.008
- VernacularTitle:静注人免疫球蛋白中IgG二聚体含量对IgG Fc段与THP-1细胞表面受体结合能力的影响
- Author:
Tingting LEI
1
;
Wei ZHANG
1
;
Changqing LI
1
;
Shengliang YE
1
;
Xi DU
1
;
Li MA
1
Author Information
1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China
- Publication Type:Journal Article
- Keywords:
IVIG;
IgG dimer;
IgG Fc fragment;
THP-1 cells;
receptor binding ability
- From:
Chinese Journal of Blood Transfusion
2023;36(2):125-129
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effect of immunoglobulin G (IgG) dimer concentration of intravenous immunoglobulin (IVIG) on the binding ability of IgG Fc fragment to THP-1 cell surface receptors. 【Methods】 Firstly, protein purification and high performance liquid chromatography (HPLC) were used to prepare different concentrations of IgG dimers. After that, IgG dimer was added to IVIG to prepare IVIG containing different concentrations of IgG dimer. Finally, based on the method established in our laboratory, we analyzed the effect of IgG dimer concentration in IVIG on the binding ability of IgG Fc fragment to THP-1 cell surface receptors. 【Results】 When the concentration of IgG dimer in IVIG was 1.11%-10.30%, its binding ability to Fc receptors on the surface of THP-1 cell was 97.67%-135.33%, and this binding ability was positively correlated with the concentration of IgG dimer. When the IgG dimer concentration exceeded 13.22%, the binding ability had no correlation with the IgG dimer concentration. 【Conclusion】 A certain concentration of IgG dimer can promote the binding ability of the IgG Fc fragment in IVIG to receptors on the surface of THP-1 cells, which needs further verification from animal experiments and clinical data.
