Analysis of ABO gene mRNA expression in peripheral blood of patients with acute myeloid leukemia
10.13303/j.cjbt.issn.1004-549x.2023.02.005
- VernacularTitle:急性髓细胞白血病患者外周血ABO基因mRNA表达分析
- Author:
Shihang ZHOU
1
;
Ni WANG
1
;
Ming LIU
2
;
Yaxin FAN
1
Author Information
1. Dalian Blood Center, Dalian 116001, China
2. Department of Cell Biology, Dalian Medical University
- Publication Type:Journal Article
- Keywords:
acute myeloid leukemia;
ABO gene;
mRNA;
TCGA database
- From:
Chinese Journal of Blood Transfusion
2023;36(2):112-115
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the expression characteristics of ABO gene mRNA in peripheral blood of patients with acute myeloid leukemia. 【Methods】 The RNA-seq data of acute myeloid leukemia in TCGA database and the whole blood RNA-seq data in GTEx database were downloaded. The difference of ABO gene mRNA expression between acute myeloid leukemia and GTEx whole blood samples was analyzed by R software, and the relationship between ABO gene mRNA expression and DNA methylation, immune infiltration and prognosis was analyzed. 【Results】 The expression level of ABO gene mRNA in acute myeloid leukemia(median: 1.333, P25: 3.654, P75: 0.401)was significantly lower than that in the control group(median: 3.576, P25: 3.747, P75: 3.470)(P<0.001). The expression level of ABO gene mRNA in acute myeloid leukemia was negatively correlated with the methylation of + 82(r=-0.249, P<0.001), + 618(r=-0.268, P<0.001), + 1 080(r=-0.105, P<0.001) and + 1 409(r=-0.210, P<0.001) at downstream of transcription start site, and positively correlated with the immune infiltration of nine types of immune cells (B cells, CD8 T cells, Cytotoxic cells, pDC, T cells, T helper cells, Tcm, Tfh and Treg) (P<0.001). There was no significant difference in overall survival between patients with high (median survival time: 366 days, confidence interval: 304-731 days) and low (median survival time: 731 days, confidence interval: 335-1 402 days) expression of ABO gene mRNA in acute myeloid leukemia (P>0.05). 【Conclusion】 The mRNA expression of ABO gene in peripheral blood of patients with acute myeloid leukemia is decreased, which is associated with DNA methylation of the first intron of ABO gene and immune infiltration, but not with the prognosis.
