Peripheral blood stem cells collection in pediatric patients with thalassemia major weighing 20 kilogram or less
10.13303/j.cjbt.issn.1004-549x.2023.06.010
- VernacularTitle:体重不超过20公斤的重型地中海贫血患儿外周血干细胞的动员和采集
- Author:
Jianyun WEN
1
;
Libai CHEN
1
;
Jing DU
1
;
Xiaoxiao XU
1
;
Yuelin HE
1
;
Yongsheng RUAN
1
;
Xiu LI
1
;
Juan LI
1
;
Xuedong WU
1
Author Information
1. Department of Pediatrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
- Publication Type:Journal Article
- Keywords:
peripheral blood stem cells;
mobilization;
collection;
20 kg or less;
thalassemia major
- From:
Chinese Journal of Blood Transfusion
2023;36(6):500-504
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To evaluate the safety and efficacy of the collection of peripheral blood stem cells (PBSCs) in pediatric patients with thalassemia major (TM) weighing 20 kg or less. 【Methods】 PBSCs collection data of 170 pediatric patients with TM weighing 20 kg or less from January 2013 to December 2020 in our center were reviewed. Safety was assessed by the occurrence of adverse events during apheresis procedures, and efficacy was evaluated by the number of CD34+ cells collected. 【Results】 A total of 171 PBSCs procedures were performed on 170 patients with TM weighing 20 kg or less, with a median age of (4.98±1.53) years and a median weight of (17.30±2.18) kg. The probability of collecting at least 1×106 CD34+ cells/kg during a single course of apheresis was 99.41% (169/170), with a median (5.88±4.23) ×106 CD34+ cells collected per kg of weight of the recipient. A minimum pre-apheresis hemoglobin (Hb) of 60 g/L in patients with TM weighing 20 kg or less was safe and feasible. The most common adverse event of G-CSF mobilization in TM patients is bone pain, with the incidence of 7.65% (13/170), which was higher than that of healthy children donors in our center. The most common adverse events during the collection were pain at the puncture site of the femoral vein (6.47%, 11/170) and low pressure of the fluid (2.92%, 5/170). And no serious complications related to PBSCs mobilization, central venous catheter(CVC)placement or the apheresis procedure occurred. 【Conclusion】 PBSCs collection by COM.TEC blood cell separator in children weighing 20 kg or less is safe and efficacious.
