Study of glycine solution in preparation of frozen thawed deglycerolized erythrocytes
10.13303/j.cjbt.issn.1004-549x.2023.07.006
- VernacularTitle:甘氨酸溶液应用于冰冻解冻去甘油红细胞制备的研究
- Author:
Daolin WANG
1
;
Hailong ZHUO
1
;
Hui LIU
1
;
Tingting CHEN
1
;
Qun LUO
1
Author Information
1. Department of Transfusion Medicine, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100071, China
- Publication Type:Journal Article
- Keywords:
glycine;
red blood cell;
ATP;
2,3-DPG;
hemolysis rate;
recovery rate
- From:
Chinese Journal of Blood Transfusion
2023;36(7):583-586
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To study the protective effect of glycine solution on frozen red blood cell thawing process. 【Methods】 A total of 20 bags of 1 U of leukocytes reduced suspended red blood cells within 6 days were selected for the study. After mixing, each 2 bags of suspended red blood cells were divided into 2 bags and into two groups with 10 bags of 1 U in each group, and were frozen for storage. One group was deglycerolized with sodium chloride solution (control group), and one group was deglycerolized with glycine solution (experimental group). The hemoglobin, free hemoglobin, residual glycerol, total glycerol in red blood cells, adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) were detected in the two groups. 【Results】 Compared with the free hemoglobin content (0.90±0.05)g/L and residual glycerol content (1.17± 0.08)g/L in the control group, the final product red blood cell supernatant free hemoglobin content (0.77±0.15)g/L and residual glycerol content (0.79±0.33)g/L in the experimental group were decreased, and the difference was statistically significant (P<0.05). Compared with the ATP content (4.03±0.38)µmol/gHb and 2,3-DPG content (485.65±78.08)µg/L in the control group, the ATP content (4.41±0.35)µmol/gHb and 2,3-DPG content (656.28±116.68)µ g/L in the experimental group were significantly increased, with statistical significance (P<0.05). 【Conclusion】 Using glycine solution instead of sodium chloride solution to prepare frozen thawed deglycerolized erythrocytes achieved the effect of protecting erythrocytes, reduced the hemolysis rate of erythrocytes and glycerin residue, and increased the recovery rate of erythrocytes.
