Viral selection pressure and CD4+T cell epitope in HLA-DRB1 * 11:01 of HCV infected patients
10.13303/j.cjbt.issn.1004-549x.2023.07.004
- VernacularTitle:HLA-DRB1 11:01与HCV感染的病毒选择压力及与CD4+ T细胞表位的关系探讨
- Author:
Ru XU
1
,
2
;
Jieting HUANG
1
,
2
;
Min WANG
1
,
2
;
Qiao LIAO
1
,
2
;
Zhengang SHAN
1
,
2
;
Huishan ZHONG
1
,
2
;
Xia RONG
1
,
2
;
Yongshui FU
1
,
2
Author Information
1. Guangzhou Blood Center, Guangzhou 510095, China
2. The Key Laboratory of Blood Safety in Guangzhou
- Publication Type:Journal Article
- Keywords:
hepatitis C virus(HCV);
positive selection sites(PSSs);
CD4+ T cell epitope;
HLA-DRB1 * 11:01;
natural outcome
- From:
Chinese Journal of Blood Transfusion
2023;36(7):571-577
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 HLA-DRB1 * 11:01, as a class HLA-Ⅱ gene, was reported to be associated with spontaneous clearance of HCV in Han and Li population. Our study was to investigate the effects of viral selection pressure and CD4+T cell epitope on the natural outcome of HCV infection in HLA-DRB1 * 11:01 positive infected patients. 【Methods】 The positive selection sites and population growth of E1E2 and NS3 genes of common HCV 6a in HLA-DRB1 * 11:01 positive and negative groups in Guangdong were respectively analyzed. The peptide library covering the conserved regions of common HCV genotypes was used to stimulate HCV spontaneous clearance group and chronic infection group using ELISPOT method. Reactive peptides were obtained according to the number of spot-forming cells per well and the frequency of occurrence in different groups. 【Results】 The positive selection sites (PSSs) of E1E2 and NS3 of common HCV 6a in HLA-DRB1 * 11:01 negative group were greater than those in HLA-DRB1 * 11:01 positive group. Furthermore, the number of PPSs in CD4+T cell peptide in HLA-DRB1 * 11:01 negative group were also greater than those in HLA-DRB1 * 11:01 positive group; Both groups of HCV 6a had a population growth in Guangdong, and the expansion trend of HLA-DRB1 * 11:01 negative group was significantly higher than that of HLA-DRB1 * 11 :01 positive group. Compared to HCV chronic infection group, the response rate of HCV spontaneous clearance group to five peptides (C-52 E2691-707, C-119 NS31545-1560, C-134 NS4A1669-1684, C-154 NS4B1912-1927, C-159 NS4B1929-1944) was higher. However, the HCV chronic infection group showed a higher response rate to two of the peptides(C-111 NS31497-1512, C-130 NS31650-1665). When HLA-DRB1 * 11:01 typing was considered, there was no significant difference in HCV-specific immune response generated by PBMCs between HLA-DRB1 * 11:01 positive and HLA-DRB1 * 11:01 negative groups. 【Conclusion】 This study revealed the relationship between viral selection pressure of HLA-DRB1 * 11:01 HCV infected persons and CD4+T cell antigen epitopes. At the same time, CD4+ T cell antigen epitopes of HCV pan-genotype were obtained, providing basic data for the development of T cell vaccine suitable for HCV pan-genotype.
