Identification of antibody against highly prevalent antigen through serological test and molecular biology technology
10.13303/j.cjbt.issn.1004-549x.2023.08.021
- VernacularTitle:血型基因检测辅助高频抗原抗体的鉴定——附一例报道
- Author:
Yulin JIANG
1
;
Xiaoping ZOU
1
;
Bujin LIU
1
;
Yun QING
1
;
Haiman ZOU
1
;
Wenjuan HUANG
1
;
Wei MAO
1
Author Information
1. Chongqing Blood Center, Chongqing 400052, China
- Publication Type:Journal Article
- Keywords:
anti-Dib;
high prevalence;
antibody identification;
gene
- From:
Chinese Journal of Blood Transfusion
2023;36(8):738-740
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To identify a case of antibody against highly prevalent antigen through molecular biology technology. 【Methods】 Blood group typing, unexpected antibody identification and cross matching were performed by serological test, and genetic testing of Diego blood group was performed by molecular biology technology. 【Results】 Serological test showed that there was a high prevalence of anti-Dib in the serum of the patient. Gene sequencing showed that the genotype of the patient was Di(a+b-) . Two cases with Di(a+b-) matched with the patient were screened from 856 blood donors. 【Conclusion】 The combined detection method based on serological test supplemented by molecular biology technology is beneficial to the detection of antibody against highly prevalent antigens, and is of great significance for ensuring the safety of clinical blood transfusion.
