Identification of B(A) subtype and analysis of bood transfusion strategiaes
10.13303/j.cjbt.issn.1004-549x.2023.08.007
- VernacularTitle:B(A)血型的鉴定及其输血策略分析
- Author:
Chunxia LIU
1
;
Lu NIU
1
;
Cuicui CHAI
1
;
Han WANG
1
;
Hongliang ZHANG
1
;
宏军 高
Author Information
1. Department of Blood Transfusion. The First Hospital of Lanzhou University, Lanzhou 730000, China
- Publication Type:Journal Article
- Keywords:
type B (A);
ABO subtype;
forward and reverse blood typing;
genotyping;
gene sequencing;
serological method;
tube method;
PCR
- From:
Chinese Journal of Blood Transfusion
2023;36(8):687-688,689
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the serology and genotype identification method of B (A) subtype patients. 【Methods】 Test tube method (serology) was used to confirm the clinically difficult ABO blood group samples of 3 patients with ABO blood group; ABO blood group was genotyped by real-time PCR, and the ABO gene exon 1-7 was sequenced to determine the genotype. 【Results】 The forward and reverse blood typing result of three patients was B (A) subtype all with ABO genotype B/O2 and c.640A> G mutation on B allele of exon 7, which meets the characteristics of ABO * BA.04 genotype. 【Conclusion】 The combination of serological and genetic testing could identify difficult blood types such as ABO subtypes accurately and ensure the safety of clinical blood use.
