Research progress of methodological techniques of generation of platelets from induced pluripotent stem cells in vitro
10.13303/j.cjbt.issn.1004-549x.2023.09.024
- VernacularTitle:诱导多能干细胞体外分化血小板技术的研究进展
- Author:
Wei YUE
1
;
Haihui GU
1
Author Information
1. Department of Transfusion Medicine, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China
- Publication Type:Journal Article
- Keywords:
thrombopoiesis;
in vitro production;
megakaryocyte (MK);
induced pluripotent stem cell (iPSC)
- From:
Chinese Journal of Blood Transfusion
2023;36(9):851-856
- CountryChina
- Language:Chinese
-
Abstract:
Platelets play a role in hemostasis in vivo, and platelet transfusion is the main means to treat bleeding diseases caused by thrombocytopenia or platelet dysfunction. However, platelets are in short supply due to the increasing demand for platelet products in clinical, the limited number of blood donors and the disadvantages of platelet products such as short shelf life and bacteria contamination. Currently, induced pluripotent stem cells are considered an ideal source for producing platelets in vitro. They have the potential for self-renewal and differentiation into any cell type, and can be obtained and manipulated easily. Given the recent advances in megakaryocytic series, bioreactors, feeder-free cell production and large-scale propagation research, platelet preparations derived from induced pluripotent stem cells have gradually shown great potential for clinical applications. Considering the minimal risk of alloimmunization and tumorigenesis with these blood products, they are promising to become the standard source of future blood transfusions. This paper reviews the research progress of the methodological techniques of in vitro generation of platelets from induced pluripotent stem cells.
