Preliminary study on HUVEC permeability changes in FNAIT induced by CD36 antibody
10.13303/j.cjbt.issn.1004-549x.2023.09.002
- VernacularTitle:CD36抗体引起的胎儿新生儿同种免疫性血小板减少症中人脐静脉内皮细胞通透性作用机理的初步研究
- Author:
Yingyi TANG
1
;
Dawei CHEN
1
;
Wenjie XIA
1
;
Xiuzhang XU
1
;
Xin YE
1
;
Bo HE
1
Author Information
1. Guangzhou Blood Center, the Key Medical Laboratory of Guangzhou, Guangzhou 510095, China
- Publication Type:Journal Article
- Keywords:
CD36 antibody;
peripheral blood mononuclear cells(PBMC);
human umbilical vein endothelial cells(HUVEC);
permeability;
fetal edema
- From:
Chinese Journal of Blood Transfusion
2023;36(9):766-770
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the pathogenesis of fetal edema caused by CD36 antibody in fetal/neonatal alloimmune thrombocytopenia (FNAIT), and to provide reference for clinical prevention and treatment. 【Methods】 The established CD36 monoclonal antibody was incubated with human peripheral blood mononuclear cells (PBMC), and the concentrations of cytokines (TNF-α and IL-1β) in the supernatant of cell culture were detected by ELISA. The permeability of endothelial cells were investigated by detecting the fluorescence intensity of FITC-albumin by incubating cytokine-rich cell supernatant with human umbilical vein endothelial cells (HUVEC). 【Results】 Flow cytometry showed that CD36 monoclonal antibody could bind to human monocytes. Compared with isotype IgG control, increased cytokine TNF-α (pg/mL) (407.73±20.40 vs 29.38 ±4.72, P<0.05) and IL-1β (pg/mL) (247.14±83.59 vs 53.68±26.96, P<0.05) were detected in the supernatant of cell culture after incubation of CD36 monoclonal antibody with human PBMC. Detection of fluorescence intensity of FITC-albumin in transwell cultured HUVEC showed that cytokine-rich cell supernatant derived from CD36 monoclonal antibody incubated with human PBMC can increase the permeability of endothelial cells significantly (CD36 antibody vs isotype IgG, MFI value: 492±16 vs 320±11, P<0.05). 【Conclusion】 The effect of CD36 monoclonal antibody on PBMC can increase HUVEC permeability, which may be one of the pathogenesis of fetal edema with FNAIT.
