Application and evaluation of human antibodies in ABO grouping
- VernacularTitle:ABO血型鉴定中人源抗体的应用与评价
- Author:
Qinqin ZUO
1
;
Liangzi ZHANG
1
;
Dazhou WU
1
;
Yong ZHANG
1
;
Xiaoyue CHU
1
;
Weiwei ZHANG
1
;
Hong WANG
1
;
Juan MAO
1
;
Hua XU
1
Author Information
- Publication Type:Journal Article
- Keywords: ABO blood grouping; human antibodies; absorption-elution test
- From: Chinese Journal of Blood Transfusion 2023;36(10):928-931
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To evaluate the application of monoclonal typing reagents and human anti-A/B antibodies for absorption-elution test in ABO grouping. 【Methods】 The specificity of monoclonal typing reagents and human anti-A/B antibodies with standard A, B, O and AB phenotypes at 4 ℃, room temperature, and 37 ℃ were compared. Affinity was evaluated by the titer, agglutination time and agglutination intensity of the reaction with A1/B cells. 29 samples with ABO discrepancy were tested to evaluate the ability of monoclonal typing reagents and human anti-A/B antibodies to detect weak antigens in absorption-elution test. 【Results】 The specificity and affinity of human anti-A/B antibodies are low, and monoclonal typing reagents have cross reactivity. Human anti-A/B antibodies can detect most weak antigens in absorption-elution test with no cross reactivity. 【Conclusion】 In ABO grouping, the human anti A/B antibody binding absorption-elution test can serve as a supplement method for identifying ABO weak antigens. Accurate results can be obtained with reasonable reagents and corresponding methodology in serological tests,thus ensuring the safety of blood transfusion.
