Detection of platelet bacteria based on cultomics and metagenomics
10.13303/j.cjbt.issn.1004-549x.2023.11.002
- VernacularTitle:基于培养组学和宏基因组学的血小板细菌检测方法研究
- Author:
Mengyi ZHAO
1
;
Anqing LIU
1
;
Yuwei ZHAO
2
;
Xia RONG
3
;
Zhengang SHAN
3
;
Zhan GAO
1
;
Yang HUANG
1
;
Miao HE
1
Author Information
1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China
2. Chengdu Blood Center
3. Guangzhou Blood Center
- Publication Type:Journal Article
- Keywords:
platelets;
metagenomics;
culturomics;
bacteria detection
- From:
Chinese Journal of Blood Transfusion
2023;36(11):978-986
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the composition of culturable bacteria in platelets through bacterial culturomics and verify the results of culturomics and metagenomics to improve the detection rate of bacteria in platelets. 【Methods】 Platelet samples from 6 healthy people were collected. Eight kinds of culture media were placed in aerobic conditions and 12 kinds of culture media were placed in anaerobic conditions for large-scale culture and isolation of bacteria in platelets. The isolated single colony was identified by 16S rRNA gene sequencing. The bacterial abundance of healthy human platelet microbiome was analyzed by metagenomic sequencing, and the cultivable bacterial species in platelets was confirmed based on metagenomic and culturomics results. 【Results】 A total of 90 strains of bacteria belonging to 3 phylums, 5 classes, 5 orders, 7 families, 9 genus and 23 species were isolated from 6 platelet samples by culturomics. Among them, the strains with more monoclonal clones at the species level were Brevundimonas aurantiaca (16.7%), Bacillus sp. Y1 (15.6%), Cutibacterium acnes (14.4%) and Brevibacillus brevis (13.3%). The platelet samples sequenced by mNGS showed that the abundance values of Proteobacteria, Firmicutes and Actinobacteria were high. The bacteria detected by both culturomics and metagenomic sequencing methods were as follows: Firmicutes: Bacillus sp. Y1, B. thuringiensis, B. cereus, B. mobilis, B. velezensis, Staphylococcus epidermidis, and Brevibacillus brevis; Actinobacteria: Cutibacterium acnes; Proteobacteria: Escherichia coli and Delftia tsuruhatensis. 【Conclusion】 The mutual validation of culturomics and metagenomics has identified some bacteria, proving that bacteria exist in platelets.
