Screening and confirmatory test of HTLV in blood donors, apreliminary study
10.13303/j.cjbt.issn.1004-549x.2021.02.016
- VernacularTitle:献血者HTLV的初筛检测及确证试验的初步研究
- Author:
Shanhai OU
1
;
Jinzhen XIE
1
;
Shucheng ZHUANG
1
;
Yongcai LIN
1
;
Bin PEI
1
;
Xiuyu SONG
1
Author Information
1. Xiamen Blood Center, Xiamen 361004, China
- Publication Type:Journal Article
- Keywords:
human T-lymphotropic virus(HTLV);
confirmatory test;
blood screening
- From:
Chinese Journal of Blood Transfusion
2021;34(2):158-161
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To study the screening and confirmatory test of human T-lymphotropic virus(HTLV) in blood donors. 【Methods】 Anti HTLV-1 / 2 screening was conducted on voluntary blood donors from 9 cities in Fujian province betweenJan. 12016toDec. 312018.Plasma samples ofanti-HTLV-1/2 reactive donors werecollected and sent to Xiamen Blood Center for confirmatory test. The influence of different screening reagents and confirmatory test methods on the test results were analyzed. 【Results】 A total of 741 anti-HTLV-1/2 reactivesamples were collected, among which 252 were positiveby Western Blot, 15undetermined, and 474negative, withthe overall positive rate at 34.0% (252/741). The yielding rate of domestic reagent A was significantly differentbyregions, withthe highest in Ningde(73.9%, 88/119) and the lowest in Zhangzhou(4.0%, 4/99). The confirmedpositive rates of anti-HTLV-1/2 screening reagent A(domestic) and B(imported)were 33.3%(13/39) and 57.1%(56/98), respectively, and the difference was statistically significant(P<0.01). When S / CO >5, the reagent Ayielding rate was 76.5%(13/17), significantly lower than that of imported reagent Bas 100%(56/56) (P<0.01). A total of 652 anti-HTLV-1 / 2 reactive samples were confirmed by Western Blot and nucleic acid test in parallel, among which 638 results were concordant, 14 were not, with the overall concordance rate at 97.85%. 【Conclusion】 Comparable differences in the yielding rate of twoanti-HTLV screening reagents were observed.There was over allhighconcordance, but also complementarity, between Western Blot and nucleic acid test.
