Activation, function and metabolismofapheresis platelets during storage
10.13303/j.cjbt.issn.1004-549x.2021.02.006
- VernacularTitle:单采血小板保存期内活化状态及相关功能和代谢指标检测及分析
- Author:
Yong YAO
1
;
Huanhuan ZHNAG
2
;
Zhenchao SUN
1
;
Yunping WU
3
;
Mengyi TANG
1
Author Information
1. Lianyungang Red Cross Central Blood Station, Lianyungang 222001, China
2. Department of Laboratory, The First People′s Hospital of Lianyungang
3. Department of Blood Transfusion, Lianyungang Second People′s Hospital
- Publication Type:Journal Article
- Keywords:
apheresis platelets;
storage;
platelet activation;
platelet function;
platelet metabolism
- From:
Chinese Journal of Blood Transfusion
2021;34(2):120-123
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the changes of activation, function and metabolism of apheresis platelets during storage. 【Methods】 Five groups of apheresis platelets(n=10per group), stored up to 5 days at 20~24℃ with agitation, were sampledat day0(4 hours within collection), 1, 2, 3 and 4, respectively to assess the activation indexes as PAC-1 and CD62P and platelet function was evaluated by thromboelastogram.The platelet counts and related parameters were determined, and the biochemical indexes such as pH, glucose, lactic acid and lactate dehydrogenase reflecting metabolism were measured and statistically analyzed. 【Results】 The CD62P (%) and PAC-1 (%) of apheresis platelets with different storage duration showed significant differences(P<0.05), and CD62P (%) increased significantly on 4thday of storage (P<0.05). There were significant differences in TEG parameters as R(min), K(min), ANG (min)and MA (mm), and MA value increased significantly after1-daystorage (P<0.05). There was no significant difference in Plt and related indexes among the groups (P>0.05). Significant differences were notable in platelet biochemical indexesby groups using variance analysis(P<0.05). The pH value and glucose were the lowest in platelets with 4-day storage, while lactic acid and lactate dehydrogenase were the highest. 【Conclusion】 The storagelesion of apheresis platelets occurs as the activation, function and metabolism of platelets developed as the storage prolonged.
