Prevalence of anti- "Mia" in blood donors and patients, Zhongshan city
10.13303/j.cjbt.issn.1004-549x.2021.02.004
- VernacularTitle:献血者和患者中抗-“Mia”筛查与分析
- Author:
Qiao LI
1
;
Ainong SUN
1
;
Shengbao DUAN
2
;
Yonglun WU
1
;
Yanting LIAO
1
;
Yuru FANG
1
;
Zhizhao YANG
3
Author Information
1. Zhongshan Blood Center, Zhongshan 528400, China
2. Suzhou Institute of Biomedical Engineering and Technology
3. Zhongshan People′s Hospital
- Publication Type:Journal Article
- Keywords:
anti-"Mia";
Mia antigen;
anti-"Mia" frequency;
salt water activity;
history of immunization
- From:
Chinese Journal of Blood Transfusion
2021;34(2):114-117
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To understand the frequency and significance of anti-" Mia" (anti-" Mia" mixtures of antibodies) in local population in Zhongshan, and the influence of different experimental conditions on the activity of human anti-" Mia" . 【Methods】 The microplate-based agglutination assay and polybrene method were used to screen anti-" Mia" in 3 587 blood samples from voluntary blood donors and patients using O type red blood cells with positive Mia antigen, then.rechecked by tube method and microcolumn gel card method. 【Results】 The frequency of anti-" Mia" was 1.06% (38/3 587), among which 60.5% (23/38) were IgM and 39.5% (15/38) were mixture of IgM and IgG; 0.61% (13/2 135) in local blood donors and 1.72% (25/1 452) in patients(P<0.01). 65.8% (25/38) of the population carrying anti-" Mia" had a history of immunity. 57.9% (22/38) were identified to be anti-" Mur" and 42.1% (16/38) anti-" Mia" using GP.Vw erythrocyte. The appropriate incubation time for anti-" Mia" test was 10 min. 【Conclusion】 The frequency of anti-" Mia" was relatively high among blood donors and patients in Zhongshan, and most of the anti-" Mia" carriers had a history of immunity. Most anti-Mia antibodies were active in saline, and some of them were mixture of IgM and IgG. It may be helpful to include Mia positive red blood cells in the irregular antibody screening cell panel to improve the safety of blood transfusion.
