Para-Bombay phenotype identification and transfusion strategy
10.13303/j.cjbt.issn.1004-549x.2021.05.026
- VernacularTitle:类孟买血型鉴定及输血策略
- Author:
Yanhua ZHANG
1
;
Lanlan CHEN
1
;
Jin CHE
1
;
Jingmin MA
1
;
Feng LI
1
;
Jiahong ZHANG
1
Author Information
1. Beijing Tongzhou Center Blood Station, Beijing 101100, China
- Publication Type:Journal Article
- Keywords:
para-Bombay blood group;
H antigen;
anti-HI;
FUT gene
- From:
Chinese Journal of Blood Transfusion
2021;34(5):541-543
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the serological and molecular biological characteristics of para-Bombay phenotype. 【Methods】 ABO blood type, H antigen and Lewis blood type in one blood sample with discrepancy between forward and reverse blood typing were detected by serological method. Antibody screening and identification and cross-match test were also performed by serological method. ABO blood group genes were detected by PCR-SSP, and FUT1 and FUT2 genes were sequenced. 【Results】 The serological test showed that the Para-Bombay phenotype was Ah secretion. The ABO blood group gene was AO2. FUT1 sequencing revealed two mutations: 235G>C and 881_882delTT. While FUT2 sequencing showed only one mutation 357C>T. 【Conclusion】 The discovery and accurate identification of blood groups is necessary to ensure the safety of blood transfusion. Blood donors of rare blood groups should be informed and recruited to the team of rare blood donors.
