ELISA detection method for IgG, IgM and IgA antibodies against hepatitis E virus: Establishment and preliminary application
10.13303/j.cjbt.issn.1004-549x.2021.05.010
- VernacularTitle:人戊型肝炎病毒IgG、IgM及IgA抗体ELISA检测方法的建立与初步应用
- Author:
Baochai LIN
1
;
Ping FU
1
;
Tiancheng LI
2
;
Caoyi LIU
1
;
Yang GAO
1
;
Yu LIU
1
Author Information
1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences& Peking Union Medical College, Chengdu 610052, China
2. Department of Virology II, National Institute of Infectious Diseases, Japan
- Publication Type:Journal Article
- Keywords:
HEV;
IgA;
blood donor;
recent infection;
ELISA
- From:
Chinese Journal of Blood Transfusion
2021;34(5):482-485
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To establish an indirect ELISA method for detecting IgG, IgM and IgA antibodies against Hepatitis E virus (HEV). 【Methods】 Tn-5 cells were infected with recombinant HEV baculovirus, and HEV-like particles (VLP) were collected and purified for coating antigen. The reaction conditions and methodology of indirect ELISA method were established and evaluated. The prevalence of HEV antibody among blood donors in Xishuangbanna were detected. 【Results】 The collected and purified VLP showed HEV antigenicity. The positive rates of anti-HEV IgG, IgM and IgA in blood donors in Xishuangbanna Prefecture were 18%(90/500), 5.6%(28/500) and 2.6%(13/500), respectively. The recent HEV infection rate was 1.8 % (9/500), and the seroprevalence of hepatitis E was 19.8% (99/500). Of the 13 anti-HEV IgA positive samples, 2 were both anti-HEV IgG and IgM negative. 【Conclusion】 HEV antibody positive is common among blood donors in Xishuangbanna, some of which are recent infections, posing a threat to the safety of blood transfusion. HEV IgA antibody indirect ELISA combined with human HEV IgM antibody detection can improve the detection rate of recent HEV infection.
