Effect of hsa_circ_0001613 on Zika virus replication: Preliminary study
10.13303/j.cjbt.issn.1004-549x.2021.05.008
- VernacularTitle:环状非编码RNA hsa_circ_0001613影响寨卡病毒复制的初步探究
- Author:
He XIE
1
;
Lan KANG
1
;
Ya ZHU
1
;
Jinghong DAI
1
;
Shilin LI
1
;
Yujia LI
1
;
Honggang SUN
1
;
Limin CHEN
1
,
2
;
Bin LI
3
;
Xiaoqiong DUAN
1
,
2
Author Information
1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu 610052, China
2. Joint Laboratory for Transfusion Transmitted Diseases between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center
3. Joint Laboratory for Transfusion Transmitted Diseases between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center
- Publication Type:Journal Article
- Keywords:
non-coding circular RNAs;
hsa_circ_0001613;
ZIKV;
virus replication;
type-ⅠIFN
- From:
Chinese Journal of Blood Transfusion
2021;34(5):472-476
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effect of transfusion-transmitted Zika virus (ZIKV) on the expression of non-coding circular RNA (hsa_circ_0001613) and the role of hsa_circ_0001613 in Zika virus replication. 【Methods】 Human adenocarcinomic alveolar basal epithelial cells (A549) were seeded on a 12-well plate at 1.8×105/ well and infected with ZIKV at 0.05 MOI. The Total RNAs were isolated every day for 5 days after infection, and the relative expression level of hsa_circ_0001613 was detected by qRT-PCR. In addition, 10nM siRNA-hsa_circ_0001613 was transfected into 2×105/ well A549 cells to specifically knock down the expression level of hsa_circ_0001613. 24h later, the cells were infected with ZIKV (MOI=0.05). Total RNAs were isolated at day 1-5 post-infection, proteins were extracted 96h post-infection. ZIKV replication, relative host antiviral gene expression, and interferon stimulated response element (ISRE) activity were tested using qRT-PCR, western blot and dual luciferase assay, respectively. 【Results】 The relative expression of hsa_circ_0001613 decreased significantly after 1-5 days of ZIKV infection. Knockdown of hsa_circ_0001613 inhibited ZIKV replication. Meanwhile, hsa_circ_0001613 knockdown significantly upregulated IFN-α/β and its downstream interferon-stimulated genes (ISGs) expression, also increased ISRE activity. 【Conclusion】 ZIKV infection significantly suppressed hsa_circ_0001613 expression in A4549 cells. Preliminary study indicated that hsa_circ_0001613 knockdown inhibited ZIKV replication possibly through activating type-Ⅰ IFN signaling pathway as showed by increased ISGs expression and ISRE activity.
