Correlation between human platelet antigen polymorphisms and platelet parameters
10.13303/j.cjbt.issn.1004-549x.2021.05.006
- VernacularTitle:血小板抗原基因多态性与血小板参数的相关性研究
- Author:
Shihang ZHOU
1
;
Ni WANG
1
;
Linnan SHAO
1
;
Weijian YU
1
;
Kaili ZHANG
2
;
Ming LIU
2
;
Xiaohua LIANG
1
Author Information
1. Dalian Blood Center, Dalian 116001, China
2. Department of Cell Biology, Dalian Medical University
- Publication Type:Journal Article
- Keywords:
human platelet antigens;
platelet parameters;
single nucleotide polymorphism
- From:
Chinese Journal of Blood Transfusion
2021;34(5):461-464
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the correlation between human platelet antigens (HPA) polymorphisms and platelet parameters. 【Methods】 The HPA-2, HPA-3, HPA-5 and HPA-15 genotypes of 139 healthy Chinese Han individuals were detected using TaqMan-MGB probe real-time PCR, while platelet parameters including platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW) and platelet-large cell ratio (P-LCR) were measured using hematology cell analyzer. 【Results】 The PLT was significantly lower in the individuals with HPA-2aa genotype compared to those with HPA-2ab [(234.35±50.10)×103/μL vs (269.58±41.66)×103/μL, P<0.05], while the PLT was significantly higher in individuals with HPA-5aa and HPA-15aa genotypes compared to those with HPA-5ab and HPA-15ab/bb [HPA-5: (239.36±49.81)×103/μL vs (200.29±48.02)×103/μL; HPA-15: (251.00±58.41)×103/μL vs (231.29±45.20)×103/μL, P<0.05], respectively. The MPV, PDW and P-LCR were significantly lower in individuals with HPA-5aa genotype compared to those with HPA-5ab [mpv: (10.01±0.72)fL vs (10.94±1.01)fL; PDV: (11.94%±1.35%) vs (14.25%±2.78%); P-LCR: (25.32%±5.03%) vs (31.73%±6.39%), P<0.05], but did not differ among the HPA-2 and HPA-15 genotypes. Besides, no significant differences in platelet parameters of individuals with HPA-3aa and HPA-3ab/bb genotypes were notable(P>0.05). HPA-2, -5 and -15 polymorphisms were identified as independent factors for platelet count, and HPA-5 polymorphism was an independent factor for platelet volume, revealed by multiple linear regression analysis. 【Conclusion】 HPA-2, -5 and -15 polymorphisms are correlated with platelet count, and HPA-5 polymorphism is correlated with platelet volume.
