Removal oftumor cells from blood by leukocyte filter
10.13303/j.cjbt.issn.1004-549x.2021.05.004
- VernacularTitle:白细胞滤器去除血液肿瘤细胞的实验研究
- Author:
Yu SHANG
1
,
2
;
Xiaobao SHAO
3
;
Lin ZHOU
3
;
Feng YAN
1
;
Peiyuan ZHU
3
Author Information
1. Department of Clinical Laboratory, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Nanjing 210009, China
2. Department of Clinical Laboratory, Nanjing Intergrated Traditional Chinese and Western Medicine Hospital Affiliated to Nanjing University of Chinese Medicine
3. Department of Transfusion Medicine, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
intraoperative cell salvage;
tumor cells removal;
leukocyte filter;
RBC recovery rate;
flow cytometry
- From:
Chinese Journal of Blood Transfusion
2021;34(5):452-455
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effect of leukocyte filter in removal of tumor cells in blood and the viability of using leukocyte filter in intraoperative cell salvage in tumor patients. 【Methods】 The leucocyte-depleted suspended RBCs prepared from 200 mL human whole blood were spiked with HCT116 cells cultured in vitro to simulate the autologous blood recovered from tumor patients during operation. Conventional filter (group A, n=6), filter with small pore size (group B, n=6) and filter with thicker membrane and small pore size (group C, n=6)) were used. The CD45-EpCAM+ tumor cells were detected by anti-CD45-PE and anti-CD326 (EpCAM)-FITC flow cytometry, and the absolute count of tumor cells were calculated using Flow Count fluorescence microspheres. The activity of tumor cells was detected by cell culture and trypan blue staining. Furthermore, blood biochemical indexes (LDH, K+ ) and red cell recovery rate were detected. 【Results】 Tumor cell counts before and after the application of leukocyte filter A, B, C were(1.52±0.48)×107/mL vs(2.73±1.74)×104/mL, (1.48±0.55)×107/ml vs(2.96±1.85)×104/mL and (1.44±0.46)×107/mL vs(3.08±2.33)×104/mL, respectively (P >0.05), which was significantly reduced after filtration (P < 0.01). No viable cells were found in the filtered blood cultured for 7 days. There were no significant differences in K+ and LDH value before and after filtration among the three groups(P >0.05). The blood recovery rates of group A, B, C were 85.22%, 84.97% and 82.86%, respectively. 【Conclusion】 The conventional filter can significantly reduce the number of circulating tumor cells, and it is feasible for intraoperative cell salvage in tumor patients.
