Emerging infectious diseases in voluntary blood donors in parts of China: Based on metagenomics analysis

Yuhui LI; Zhan Gao; Shilin LI; Yujia LI; Yang Huang; Limin Chen; Mei Huang; Jianhua Wan; Weilan HE; Wei Mao; Jie Cai; Jingyu Zhou; Ru Yang; Yijing Yin; Yanli Guo; Miao HE

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Emerging infectious diseases in voluntary blood donors in parts of China: Based on metagenomics analysis

VernacularTitle:基于中国部分地区无偿献血人群宏基因组学的新发病原体分析
Author: Yuhui LI ^{1
,

2} ; Zhan GAO ¹ ; Shilin LI ¹ ; Yujia LI ¹ ; Yang HUANG ¹ ; Limin CHEN ¹ ; Mei HUANG ³ ; Jianhua WAN ⁴ ; Weilan HE ⁵ ; Wei MAO ⁶ ; Jie CAI ⁷ ; Jingyu ZHOU ⁸ ; Ru YANG ⁹ ; Yijing YIN ¹⁰ ; Yanli GUO ¹¹ ; Miao HE ¹
Author Information

1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China
2. Sichuan Blood Safety and Blood Substitute, International Science and Technology Cooperation Base
3. The Red Cross Central Blood Station of Mianyang
4. Blood Center of Urumqi
5. Blood Center of Guangxi Zhuang Autonomous Region
6. Blood Center of Chongqing
7. The Red Cross Blood Center of Nanjing
8. Blood Center of Jiangsu Province
9. Blood Center of Wuhan
10. Central Blood Station of Dehong Prefecture
11. Central Blood Station of Mudanjiang
Publication Type:Journal Article
Keywords: emerging pathogens; voluntary blood donors; China; metagenomics; high-throughput sequencing; emerging infectious diseases; bioinformatics
From: Chinese Journal of Blood Transfusion 2021;34(5):440-446
CountryChina
Language:Chinese
Abstract: 【Objective】 To analyze the metagenomics and microbiology of voluntary blood donors in China, so as to assess the potential threats of emerging infectious diseases to the safety of blood transfusion. 【Methods】 12 300 plasma samples (10 mL each) collected by central blood stations in Chongqing, Liuzhou, Urumqi, Mianyang, Wuhan, Nanjing, Mudanjiang, and Dehong Prefecture area from 2012 to 2018 were subjected to total DNA extraction after ultracentrifugation (32 000 rpm/min, centrifugal radius 91.9 mm) in minipools of 160 donations. The metagenomic library was constructed, and deep sequencing was conducted by Illumina Hiseq 4 500. By comparing with reference sequences of bacteria, fungi, parasites and viruses, metagenomic data were analyzed, classification of microbes were identified, and potentially harmful pathogens were evaluated. 【Results】 A total of 632 GB clean data were obtained by deep sequencing, and the top three pathogens were Pseudomonas(0.561 1%), Burkholderia(0.468 7%) and Serratia(4.242 0%). Pathogens with potential threat which could be transmitted by blood transfusion or blood products were found, such as human parvovirus B19(0.126 6%), Leishmania spp(1.348 5%) and Toxoplasma gondii(0.615 8%). 【Conclusion】 Our study analyzed metagenomics of voluntary blood donors in parts of China and revealed pathogens that may cause potential harm to blood safety, which were helpful for targeted prevention and control of emerging infectious diseases.