Monoclonal anti-CD47 interference in pre-transfusion test and mitigation measures, a case report
10.13303/j.cjbt.issn.1004-549x.2021.03.024
- VernacularTitle:抗-CD47单克隆抗体对输血前检测试验的干扰及处理措施
- Author:
Chunyan MO
1
;
Yuzhen CEN
2
;
Zhen WANG
1
;
Ling WEI
1
;
Yanli JI
1
;
Guangping LUO
1
Author Information
1. Institution of Clinical Blood Transfusion, Guangzhou Blood Center, Guangzhou 510095, China
2. Transfusion Department, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen university
- Publication Type:Journal Article
- Keywords:
anti-CD47;
red blood cell transfusion;
IgG4 deficiency;
anti human globulin
- From:
Chinese Journal of Blood Transfusion
2021;34(3):290-292
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To discuss the interference of anti-CD47 in pre-transfusion test and the mitigation measures. 【Methods】 Blood sample of one patient received anti-CD47 treatment was collected to conduct routine serological tests including ABO/Rh phenotype, direct anti-human globulin test, irregular antibody screening, antibody identification and cross-match. Packed platelet from multiple type O blood donors was used to absorb with patient′s plasma. The patient′s plasma was absorbed with CCDee, ccDEE and ccdee red cells, respectively. Anti-IgG monoclonal Gamma-clone which lacks reactivity with human subclass IgG4 was used to perform antibody screening and cross-match. Capture-R was used to perform antibody screening. 【Results】 The direct anti-human globulin test was positive(1+ ), the reactivity in all phases was strong positive(3+ -4+ ). The anti-CD47 was eliminated after platelet and red cells absorption. Antibody screening became negative using Gamma-clone and Capture-R, and cross-match successfully using Gamma-clone. 【Conclusion】 Anti-CD47 monoclonal antibody can interfere with pre-transfusion test and cross matching. To remove the interference of anti-CD47 requires the use of Gamma-clone anti-IgG in the indirect antiglobulin testing or Capture-R.
