Distribution and haplotype polymorphism of HLA-A、-B、-C、-DRB1、-DQB1 alleles in Anhui Han population
- VernacularTitle:安徽省汉族人群HLA-A、-B、-C、-DRB1、-DQB1等位基因分布及单体型多态性研究
- Author:
Chao WANG
1
;
Xiaoyu HU
1
;
Yang ZHAO
1
;
Juan YU
1
;
Huixin JIN
1
;
Jiayu WEI
1
;
Feifei JIANG
1
;
Nana ZHAO
1
;
Chaoqun GU
1
;
Rong LV
1
Author Information
- Publication Type:Journal Article
- Keywords: HLA; high resolution genotyping; haplotype frequency; allele
- From: Chinese Journal of Blood Transfusion 2021;34(3):240-244
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To study the distribution and haplotype polymorphism of HLA-A, -B, -C, -DRB1, -DQB1 alleles in Anhui Han population. 【Methods】 The HLA-A, -B, -C, -DRB1 and -DQB1 genotyping of 3 169 random unrelated stem cell donors was performed by PCR-SBT. The allele frequency, haplotype frequency and linkage imbalance parameters were calculated by counting method, maximum expectation algorithm and PyPop software. 【Results】 A total of 411 HLA alleles were detected in the population, of which 67, 143, 65, 75 and 64 alleles were detected for HLA-A, -B, -C, -DRB1 and -DQB1, respectively. The alleles with frequency >0.1 were HLA-A*11∶01, A*11∶01, A*24∶02, A*02∶01, C*01∶02, C*07∶02, C*06∶02, DRB1*09∶01, DRB1*15∶01, DRB1*07∶01, DQB1* 03∶01, DQB1* 03∶03, and DQB1*02∶01. 1426 HLA-A~HLA-B, 1 772 HLA-B~HLA-DRB1, 798 HLA-B~HLA-C, and 446 HLA-DRB1~HLA-DQB1 haplotypes were detected. The haplotypes showed linkage imbalance, and 19 of them showed strong linkage imbalance (RLD>0.80). 【Conclusion】 The frequency and haplotype distribution of HLA-A, -B, -C, -DRB1 and -DQB1 alleles in Anhui Han population were obtained. The distribution of those alleles and haplotypes have their own characteristics.
