Correlation between the polymorphism of erythrocyte membrane blood group glycoprotein A (GPA) related gene GYPA and clonorchis sinensis infection
10.13303/j.cjbt.issn.1004-549x.2021.03.006
- VernacularTitle:红细胞膜血型糖蛋白A(GPA)相关基因GYPA多态性与华支睾吸虫感染的相关性研究
- Author:
Xiongchi TANG
1
;
Qingping XU
1
;
Xiaorong WEI
2
;
Lewen ZHANG
1
;
Zhiyong JIANG
3
;
Yong LU
1
;
Jianfeng SU
1
;
Yanlian LIANG
4
Author Information
1. People′s Hospital of Pingnan County, Guigang 537300, China
2. Wuming Hospital of Guangxi Medical University
3. Guigang People′s Hospital
4. Institute of Transfusion Medicine, Shenzhen Blood Center
- Publication Type:Journal Article
- Keywords:
erythrocyte membrane blood group glycoprotein;
clonorchis sinensis;
infection;
genetic mutation;
disease correlation
- From:
Chinese Journal of Blood Transfusion
2021;34(3):223-226
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the polymorphism of erythrocyte membrane blood group glycoprotein A (GPA) related gene GYPA in high and low endemic population for clonidia sinensis infection, aimed at investigating the correlation between erythrocyte transmembrane glycoproteins and clonorchis sinensis infection. 【Methods】 From Dec 2019 to Jun 2020, anticoagulant blood samples were randomly collected in WuMing district (n=700) and GuiGang district (n=500 ) of Nanning city, and the IgG antibody to clonorchis sinensis in plasma was detected, and the DNA of leukocyte was extracted. The full-length exon and partial intron of GYPA gene were sequenced, mutations were characterized by gene cloning, and the risk of infection was calculated by chi-square test. 【Results】 The yield rate of IgG antibody was 62.7% (439/700) in WuMing district and 3.4% (17/500) in GuiGang district(P<0.05). The insertion of base C at the 54th position of intron-2 in GYPA gene caused the reading frame shift. The mutation was presented in 23.9% (105/439) and 17.6% (3/17) of the population with clonorchis sinensis exposure in WuMing and GuiGang area, respectively, while 49.4% (129/261) and 54.7% (264/483) in the negative population, respectively (P<0.05). 【Conclusion】 The infection rate of clonorchis sinensis in WuMing district was higher than that in GuiGang district. The mutation rate of reading frame shift caused by the insertion of base C at the 54th position of GYPA intron-2 was much lower in the positive population of clonorchis sinensis infection than the negative population, suggesting that the mutation is a protective gene in the negative population of clonorchis sinensis infection. It is necessary to study the mechanism of clonorchis sinensis infection and the mutation point of this gene in order to facilitate the early diagnosis of disease, blood transfusion management, treatment and prevention.
