Absorption and elution test and real time PCR in ABO blood group identification: A comparative study
10.13303/j.cjbt.issn.1004-549x.2021.04.022
- VernacularTitle:吸收放散试验与实时荧光定量PCR在ABO血型鉴定中的性能评价
- Author:
Xiaofei LI
1
;
Tianhong MIAO
1
;
Chongyun SUN
1
;
Tingting LIU
1
Author Information
1. Beijing Red Cross Blood Center, Beijing 100088, China
- Publication Type:Journal Article
- Keywords:
ABO blood group;
absorption and elution test;
real time PCR;
gene sequencing
- From:
Chinese Journal of Blood Transfusion
2021;34(4):408-411
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To evaluate the performance of absorption and elution test and real time PCR in identifying ABO blood group. 【Methods】 The blood samples with weakened antibody expression serologically were screened and analyzed by absorption and elution test, real time PCR and gene sequencing. The gene sequencing results were used as the gold standard to assess the consistency of the other two methods, and the sensitivity and specificity were compared. 【Results】 Compared with the sequencing results (gold standard), absorption and elution test showed moderate consistency, while real time PCR showed good consistency. Both the sensitivity and specificity of absorption and elution test and real time PCR were statistically different. 【Conclusion】 There was a good agreement between real time PCR and gene sequencing in identifying suspicious ABO blood group with weakened antibody expression serologically. Real time PCR, with a low misjudgment rate, has strong ability to detect weak antigen and is more conducive to identify blood type compared with absorption and elution test.
