Genotyping of HPA-1-29bw in platelet donors in Nanjing, China
10.13303/j.cjbt.issn.1004-549x.2021.04.006
- VernacularTitle:南京地区血小板捐献者HPA-1-29bw基因分型检测
- Author:
Ruoyang ZHANG
1
;
Rong CHEN
1
;
Taixiang LIU
1
;
Li CAI
1
;
Wenbiao LIANG
1
Author Information
1. Jiangsu Province Blood Center, Nanjing 210042, China
- Publication Type:Journal Article
- Keywords:
human platelet antigens;
HPA gene;
gene frequency;
platelet donors
- From:
Chinese Journal of Blood Transfusion
2021;34(4):349-353
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the allele frequencies of the human platelet antigens 1-29 system (HPA-1-29bw) in Nanjing Han platelet donors, so as to provide references for compatible platelet transfusion. 【Methods】 HPA genotyping was performed by Sanger sequencing method in 900 Nanjing Han regular platelet donors who donated at Jiangsu Province Blood Center from February to September 2019. The frequencies of alleles and genotype were calculated using direct counting method. 【Results】 The HPA allele frequencies in Nanjing Han platelet donors were HPA-1a 0.9950, 1b 0.0050, 2a 0.9467, 2b 0.0533, 3a 0.5850, 3b 0.4150, 4a 0.9989, 4b 0.0011, 5a 0.9822, 5b 0.0178, 6a 0.9828, 6b 0.0172, 11a 0.9994, 11b 0.0006, 15a 0.5317, 15b 0.4683, 21a 0.9928 and 21b 0.0072, respectively. Only a allele was detected in HPA-7-10w, -12-14w, -16-20w and -22-29bw systems.The highest mismatch rate of HPA genes in 900 platelet donors was HPA-15 system, followed by HPA-3 system, with the rate of 37.40%(337/900) and 36.77%(331/900), respectively. One heterozygote was detected in HPA-11w system. 【Conclusion】 The chracteristics of HPA alleles frequencies in Nanjing Han platelet donors is that HPA-15 and HPA-3 are the most common heterozygotes, which should be paid attention to in local clinical transfusion.
