Effect of sample processing at 56℃ for 30 min on routine examination in Department of Blood Transfusion
10.13303/j.cjbt.issn.1004-549x.2021.04.005
- VernacularTitle:56℃30 min灭活病毒方法对输血检验的影响
- Author:
Bingxiang ZHAN
1
;
Yanhong CHENG
1
;
Chunqiu LIU
1
;
Peng YANG
1
;
Maohong BIAN
1
Author Information
1. Department of Blood Transfusion, the First Affiliated Hospital of Anhui Medical University, Heifei 230022, China
- Publication Type:Journal Article
- Keywords:
virus inactivation;
heating treatment;
routine examination in Department of Blood Transfusion;
blood type;
antibodies;
cross-matching
- From:
Chinese Journal of Blood Transfusion
2021;34(4):346-349
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effect of sample processing at 56℃ for 30 min on routine examination in Department of Blood Transfusion. 【Methods】 A total of 40 cross matched blood samples submitted by clinical departments of our hospital, were collected, and each sample was equally divided into two. Before and after heating at 56℃ for 30 min, the ABO blood group was detected by manual method and card method (gel card and glass beadle card), antibody titer was detected by coagulant method, and cross-matching was conducted by anti-globulin card method. Chi-square test and Wilcoxon signed rank test were used to compare the differences between the two groups (before and after heating treatment). 【Results】 The blood group detection rates of the experimental group were 100% (40/40), 37.5% (15/40) and 80% (32/40) by manual test tube method, gel card and glass beads card, respectively, P<0.01. The geometric mean of blood antibody titers in the control group and the experimental group were anti-A IgM (14.936, 10.556) and IgG(3.563, 4.489), anti-B IgM (9.849, 10.556) and anti-G (5.039, 4.489)(respectively, P>0.05). The matching rate of two groups of samples, cross-matched with corresponding donor samples, was both 100% (40/40) by coagulant method, and 100% (40/40) vs 25% (10/40) respectively by the antiglobulin card method (P<0.01). The other 30 samples in the experimental group presented weak agglutination in the secondary side. 【Conclusion】 The treatment of virus inactivation at 56℃ for 30 min has little effect on blood group identification by test tube method, antibody titer and cross-matching by coagulant method, and reduceds the occupational exposure of staff in Blood Transfusion Department.
