Genetic polymorphism and antibody screening of RhD negative Chinese Han population in Jiayuguan
10.13303/j.cjbt.issn.1004-549x.2021.06.020
- VernacularTitle:嘉峪关市RhD阴性汉族人群基因多态性及抗体筛选调查
- Author:
Ying WANG
1
;
Jiewei ZHENG
2
;
Yanhong CUI
1
;
Yue YANG
1
;
Jinyu CHEN
1
;
Jin DANG
1
;
Xiaodan LIU
1
;
Junhua XIE
2
;
Dong XIANG
2
Author Information
1. Jiayuguan Blood Station, Jiayuguan 735100, China
2. Shanghai Blood Center
- Publication Type:Journal Article
- Keywords:
RhD negative;
D-variant;
gene sequencing;
anti-D
- From:
Chinese Journal of Blood Transfusion
2021;34(6):642-645
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate and analyze the polymorphism of RHD gene in RhD-negative population in Jiayuguan using molecular biological technique, so as to accurately identify RhD-negative individuals, and formulate individualized transfusion strategies. 【Methods】 The RhD negative voluntary blood donors and patients (mainly pregnant women) were recruited. After informed consent, history of blood transfusion and pregnancy of them were investigated, and samples were collected for negative D confirmation, gene sequencing as well as antibody screening and identification. 【Results】 Among the 96 samples, 73 cases were RHD gene deletion, 18 RHD*01EL.01(17 RHD1227A homozygous type and 1 RHD1227A heterozygous type), 2 weak RHD*15 type (845G/A), 1 partial D type, i. e. RHD-CE(7) -D heterozygous allele, and 2 RHD*01N.16 variant. Antibody was detected out in 4 cases, among which 2 were positive for anti-D, 1 anti-D plus anti-E, and 1 anti-Dia. 【Conclusion】 The proportion of DEL gene in RhD negative Chinese Han population in Jiayuguan is slightly lower than that in general Chinese Han population. No anti-D or RHD-HDN was observed in DEL type women due to multiple pregnancy or delivery of D positive newborns.
