Correlation between hemolysis degree and antibody IgG subtypes contained in infant serum and erythrocyte eluates
10.13303/j.cjbt.issn.1004-549x.2021.06.015
- VernacularTitle:ABO新生儿溶血患儿血浆游离及红细胞放散抗体IgG亚型与溶血程度的相关性分析
- Author:
Jin YANG
1
;
Jiehua CHU
1
;
Mingpeng CAO
1
;
Lingbo LI
2
;
Jing ZHONG
3
Author Information
1. Department of Blood Transfusion, Hefei Second People's Hospital of Anhui Province, Hefei 230011, China
2. R&D Department of Changchun Boxun Biotechnology Co., Ltd.
3. Department of Blood Transfusion, Shaoyang Central Hospital of Hunan Province
- Publication Type:Journal Article
- Keywords:
three hemolysis items;
plasma IgG1 and IgG3 antibodies;
degree of hemolysis;
the regression equation
- From:
Chinese Journal of Blood Transfusion
2021;34(6):624-627
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the effects of IgG subtypes(IgG 1 and IgG3) of antibodies contained in infant serum and erythrocyte eluates on hemolytic disease of the newborn(HDN), so as to provide reference for its early clinical diagnosis and treatment. 【Methods】 49 newborns with HDN in our hospital from June 2019 to March 2020 were detected for three hemolytic tests(direct antiglobulin test, elution test and indirect antiglobulin test), as well as the components of IgG1 and IgG3 in eluates. The correlation analysis was conducted by combining birth hours (physiological jaundice) and hemolytic degrees (total bilirubin, indirect bilirubin, and hemoglobin). 【Results】 In the 44 cases of IgG1 and IgG3 subtype detection of infant RBC eluates, regression equations could be established between total bilirubin, indirect bilirubin and birth hours, and between hemoglobin and elution test, and linear regression relationships were found (P<0.05). In the 28 cases of IgG1 and IgG3 subtype detection of infant serum, regression equations could be established between total bilirubin, indirect bilirubin, birth time and IgG3 subtype, and between hemoglobin and IgG1 subtype (P<0.05), and linear regression relationships were found (all P<0.05). Three infants, presenting IgG1 and IgG2 subtypes(+ ) and three hemolysis tests(-), were all second pregnancy, constituted by Rh-HDN of 2 case and other-system-HDN 1. 【Conclusion】 The degree of HDN is directly related to IgG1 and IgG3 antibodies in infant blood plasma. In addition to the total bilirubin and indirect bilirubin, the changes of IgG3 antibodies in infant plasma and IgG1 antibody in anemic infants should be monitored. If IgG1 and IgG3 antibodies are yielded even with all negative ABO-HDN hemolysis tests, non-ABO-HDN should be considered in time to achieve accurate diagnosis and treatment.
