Phenotype screening and gene mutation analysis of platelet CD36 antigen: blood donors in Suzhou area

Honghong HE; Jing Chen; Yezhou Chen; Yujue Wang; Longhai Tang; Hongmei Wang; Shaohua Ding; Shuangshi Wei; Jingjing Tian; Shengbao Duan

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Phenotype screening and gene mutation analysis of platelet CD36 antigen: blood donors in Suzhou area

VernacularTitle:苏州地区献血者血小板CD36抗原表型筛查及基因突变分析
Author: Honghong HE ¹ ; Jing CHEN ¹ ; Yezhou CHEN ² ; Yujue WANG ² ; Longhai TANG ¹ ; Hongmei WANG ² ; Shaohua DING ² ; Shuangshi WEI ² ; Jingjing TIAN ² ; Shengbao DUAN ²
Author Information

1. Suzhou Blood Center, Suzhou 215006, China
2. Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences
Publication Type:Journal Article
Keywords: CD36 antigen; deficiency; gene mutation; database
From: Chinese Journal of Blood Transfusion 2021;34(6):585-589
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the expression of CD36 antigen in Suzhou area, analyze the type of antigen deficiency and gene mutation, so as to provide references for the establishment of CD36 negative donor registry in Suzhou. 【Methods】 Anticoagulant whole blood samples (805 cases) were randomly collected from healthy blood donors in Suzhou Blood Center. The expression of CD36 antigen on platelet and monocyte was analyzed by flow cytometry to determine the type of CD36 deficiency. The gene mutation type of platelet CD36 antigen-deficient was performed by genomic DNA sequencing. 【Results】 The CD36 deficiency frequency on platelet was 2.48% (20/805), among which TypeⅠ(lacking CD36 expression both on platelet and monocyte) and TypeⅡ(lacking CD36 expression on platelet only) CD36 deficiency accounted for 10% (2/20) and 90% (18/20), respectively. CD36 gene mutations were found in 10 samples, including 3 cases of 329_330 d^elAC, 1 case of 1228_1239 d^elATTGTGCCTATT and 2 cases of 1163 A>T; 1 case of 329_330 d^elAC+ 1172_1183 d^elTATTGGTCAAGC and 287 G>C+ 329_330 d^elAC heterozygous mutation. In addition, 1 case of 745 A>G and 1 case of 806 C>T mutations were novel, and not yet reported. 【Conclusion】 Results showed that the frequency of CD36 antigen deficiency in Suzhou were similar to that reported in southern China, but the mutation sites were slightly different. The establishment of CD36 negative platelet registry could provide negative platelets for patients with transfusion reactions caused by anti-CD36 antibody and improve the effect of clinical platelet transfusion.