Thromboelastogram parameters and MTHFR gene polymorphism in the diagnosis of adverse fetal pregnancy outcomes
10.13303/j.cjbt.issn.1004-549x.2021.07.014
- VernacularTitle:血栓弹力图参数指标与MTHFR基因多态性在胎儿不良妊娠结局中的诊断价值
- Author:
Jing QIAO
1
;
Xinfeng PANG
2
;
Rencun WANG
3
;
Xin ZHANG
4
;
Ailing WANG
3
Author Information
1. Department of Pediatrics, Jiaozuo Maternal and Child Health Hospital, Jiaozuo 454000, China
2. Department of Blood Transfusion, Jiaozuo Maternal and Child Health Hospital, Jiaozuo 454000, China
3. Department of Obstetrics, Jiaozuo Maternal and Child Health Hospital, Jiaozuo 454000, China
4. Department of Medical Genetics and Antenatal Diagnosis, Jiaozuo Maternal and Child Health Hospital, Jiaozuo 454000, China
- Publication Type:Journal Article
- Keywords:
adverse pregnancy outcome;
methylene tetrahydrofolate reductase(MTHFR);
C677T;
thrombus elastogram(TEG);
MA value
- From:
Chinese Journal of Blood Transfusion
2021;34(7):728-731
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To retrospectively analyze the situation of patients with adverse fetal outcomes by thromboelastogram (TEG) parameters and, MTHFR gene polymorphism, so as to provide molecular biological diagnosis basis for patients with adverse pregnancy outcomes, and a new scheme for early prevention and treatment of women of childbearing age with MTHFR gene polymorphism. 【Methods】 A total of 100 women with adverse fetal pregnancy outcomes were selected as the adverse pregnancy group, and 100 healthy women of childbearing age with normal pregnancy history were selected as the controls. MTHFR gene C677T and A1298C polymorphisms were detected by polymerase chain reaction (PCR). TEG and blood coagulation were detected in the experimental group. 【Results】 The A1298C gene polymorphism(AA、CC、AC; A、C) was similar in both adverse pregnancy group and the controls. The frequency distribution of C, T allele of MTHFR gene C677T was statistically significant (χ2=4.60, P<0.05, OR =1.645, 95% CI: 1.042~2.595). TT and CT+ CC types showed significant different association with the factors of stillbirth(χ2 =7.49, P<0.05). MA value of TEG in the diagnosis of TT type of C677T genotypes MTHFR in 32 patients with adverse pregnancy outcome was analyzed. The area under the AUC curve of MA value was 0.795. 【Conclusion】 MTHFR C677T polymorphism TT with TEG parameter hypercoagulability is an important risk factor in the occurrence of pregnancy stillbirth in adverse pregnancy outcomes.
