Serological and genotyping results of ABO subtypes in tumor patients
10.13303/j.cjbt.issn.1004-549x.2021.07.009
- VernacularTitle:肿瘤患者ABO亚型血清学与基因分型检测结果分析
- Author:
Xiying LI
1
;
Junhua HU
2
;
Juan CAI
1
;
Tao PENG
1
;
Jingjing YU
1
;
Min LIU
1
;
Guohua ZHAO
1
Author Information
1. Department of Blood Transfusion, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
2. Department of Blood Transfusion, Beijing Hospital, National Center of Gerontology
- Publication Type:Journal Article
- Keywords:
tumor patients;
ABO subtypes;
serology;
genotyping
- From:
Chinese Journal of Blood Transfusion
2021;34(7):708-712
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze ABO subtype samples from tumor patients using both blood group serology and molecular biology technology. 【Methods】 32 samples of ABO subtype, identified by routine blood group serology in the Department of Transfusion, of solid tumor patients treated in Cancer Hospital of Chinese Academy of Medical Sciences from July 2014 to December 2019 were analyzed by sequence specific primer polymerase chain reaction (PCR-SSP) and gene sequencing (PCR-SBT). 5 samples were detected by PCR-SSP, 28 PCR-SBT, 1 both PCR-SSP and PCR-SBT. The serological and genotyping results were comparatively analyzed. 【Results】 66% (21/32) of the samples in this study were confirmed as ABO subtypes or harbored mutations by genotyping assays, including 7 cases of B(A).04, 2 of B(A).02, 1 of cis AB.01, 3 of BW.12, 4 of BEL.03, 1 of AEL.02, 1 of B 974G>C, 1 of A 797insT, and 1 of A 617C>G. Among the 21 subtype samples or harbored mutations, 15 had the same blood group serology and genotyping results, and 6 had no A or B antigen detected in the serological adsorption-elution test. An additional 34% (11/32) of the samples had no variants in the gene responsible for ABO subtype detected by molecular analysis. 【Conclusion】 Molecular biology techniques can assist in the identification of difficult ABO blood types caused by weakened antigens or antibodies, and subtypes in solid tumor patients, which is an important auxiliary method for accurate identification of difficult ABO blood group.
