Single-ELISA reactive rate of four blood screening items in 18 blood station laboratories: Henan, 2019

Yan Zhang; Mingjun Chen; Hongna Zhao; Yonglei LV; Jianhua Fang; Qi Jiang; Shanhua Chen; Dan Chen; Jia WU; Nan Chen; Yu Meng; Huiyun Han; Dengfeng Wang; Xiaozhen Zhang; Yue Zhang; Fengyong MA; Bin Jia; Junjie LI; Sili Sun; Fei Zhao; Yongqing Zhou; Suneng Zhang

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Single-ELISA reactive rate of four blood screening items in 18 blood station laboratories: Henan, 2019

VernacularTitle:河南省18家血站实验室2019年血筛4项单试剂反应率调查与分析
Author: Yan ZHANG ¹ ; Mingjun CHEN ¹ ; Hongna ZHAO ¹ ; Yonglei LV ¹ ; Jianhua FANG ¹ ; Qi JIANG ² ; Shanhua CHEN ³ ; Dan CHEN ⁴ ; Jia WU ⁵ ; Nan CHEN ⁶ ; Yu MENG ⁷ ; Huiyun HAN ⁸ ; Dengfeng WANG ⁹ ; Xiaozhen ZHANG ¹⁰ ; Yue ZHANG ¹¹ ; Fengyong MA ¹² ; Bin JIA ¹³ ; Junjie LI ¹⁴ ; Sili SUN ¹⁵ ; Fei ZHAO ¹⁶ ; Yongqing ZHOU ¹⁷ ; Suneng ZHANG ¹⁸
Author Information

1. Henan Red Cross Blood Center, Zhengzhou 450052, China
2. Kaifeng Central Blood Station
3. Luoyang Central Blood Station
4. Pingdingshan Central Blood Station
5. Anyang Central Blood Station
6. Hebi Central Blood Stationr
7. Xinxiang Central Blood Station
8. Jiaozuo Central BloodStation
9. Puyang Central Blood Station
10. Xuchang Central Blood Station
11. Luohe Central Blood Station
12. Sanmenxia Central Blood Station
13. Shangqiu Central Blood Station
14. Zhoukou Central Blood Station
15. Zhumadian Central Blood Station
16. Nanyang Central Blood Station
17. Xinyang Central Blood Station
18. Jiyuan Central Blood Station
Publication Type:Journal Article
Keywords: ELISA; Henan; blood station laboratory; quality management
From: Chinese Journal of Blood Transfusion 2021;34(8):878-882
CountryChina
Language:Chinese
Abstract: 【Objective】 To analyze the cause of single-ELISA reactive of four blood screening items in 18 blood stations in Henan, so as to provide the basis for improving the quality of blood screening. 【Methods】 The single-ELISA reactive rate of HBsAg, anti-HCV, HIV Ag/Ab and anti-TP of 18 blood station laboratories in Henan throughout 2019 was calculated, and the causes were analyzed according to different ELISA reagent combinations and gray area settings in each laboratory. 【Results】 The overall single-ELISA reactive rates of HBsAg, anti-HCV, HIV Ag/Ab and anti-TP were 1.740(2 154/1 237 789), 0.564‰(698/1 237 789), 1.421‰(1 759/1 237 789) and 1.561‰(1 932/1 237 789), respectively, showing significant differences by detection items (P <0.05). Person correlation analysis showed that the single-ELISA reactive rate was independent of the gray area settings.but dependent on laboratories and reagent combinations. The single-ELISA reactive rate of HBsAg, anti-HCV, HIV Ag/Ab and anti-TP in D laboratory was the highest and higher than that in other labs using the same reagent.The laboratories with high HBsAg single-ELISA reactive rate were mostly those using a combination of imported reagents and domestic reagents, including the top 6 laboratories. The laboratories with high anti-HCV single-ELISA reactive rate were mostly those using certain domestic reagents. No obvious rules was noticed by single-ELISA reactive for anti-HIV. Laboratories with high anti-TP single-ELISA reactive rate were mostly those using combination 4. 【Conclusion】 The HBsAg single-ELISA reactive rate was the highest in the four blood screening items of blood station laboratories in Henan. The single-ELISA reactive rate is related to the laboratory itself and the reagent manufacturer, suggesting that laboratory quality control should be strengthened and proper reagent combination should be selected to reduce the waste of blood.