Preparation and clinical application of 0.7~0.8 hematocrit concentrated washed red blood cells in intrauterine blood transfusion for fetal anemia

Xiaomin Huang; Yanli JI; Qun Fang; Bo HE; Sihai Zeng; Kejun Huang; Ailing Tan; Hanmei Chen; Wenxiu Cui; Wenfang Zhang

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Preparation and clinical application of 0.7~0.8 hematocrit concentrated washed red blood cells in intrauterine blood transfusion for fetal anemia

VernacularTitle:胎儿贫血行宫内输血术时0.7～0.8 Hct浓缩洗涤红细胞制备技术开发与临床应用
Author: Xiaomin HUANG ¹ ; Yanli JI ¹ ; Qun FANG ² ; Bo HE ¹ ; Sihai ZENG ¹ ; Kejun HUANG ¹ ; Ailing TAN ¹ ; Hanmei CHEN ¹ ; Wenxiu CUI ¹ ; Wenfang ZHANG ¹
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1. Guangzhou Blood Center, Guangzhou Medical Key Laboratory, Key Laboratory of Blood Safety, Guangzhou 510095, China
2. Fetal Medical Center, The First Affiliated Hospital of Sun Yat-sen University
Publication Type:Journal Article
Keywords: anemia; intrauterine blood transfusion; hematocrit; washed red blood cells
From: Chinese Journal of Blood Transfusion 2021;34(9):1000-1002
CountryChina
Language:Chinese
Abstract: 【Objective】 To develop a new approach for the preparation of 0.7~0.8 hematocrit concentrated washed red blood cells(RBCs) for fetal anemia in utero transfusion and apply it in clinical. 【Methods】 The erythrocyte suspension and frozen stored erythrocytes within expiry date in Guangzhou Blood Center from March 2020 to February 2021 were taken to prepare concentrated washed RBCs. According to the derivation formula, corresponding weight of RBC preservation solution was added to obtain 0.7~0.8 hematocrit concentrated washed RBCs. Routine blood test data were statistically analyzed by single-sample t test, and P<0.05 was considered statistically significant. Qualified Rh-negative/ O-type 0.7~0.8 hematocrit concentrated RBCs within expiry date were used in clinical intrauterine blood transfusion. 【Results】 The hematocrit of concentrated washing RBCs prepared by the new approach could reach 0.7~0.8. The RBCs count (8.389 ±0.808)×1 0¹²/ L and hemoglobin content(233.730±15.498)g/L were higher while the erythrocyte count (0.732±0.469)×10⁹ /L and platelets count(26.000±26.276)×10⁹/L were lower than the normal values of adults. The mean erythrocyte volume(fL), hemoglobin content(pg) and concentration(g/L )were 88.123±6.359, 30.004±2.809 and 339.980±11.865, respectively, which were normal values of adults. Fetal anemia was significantly improved and the prognosis was good after intrauterine blood transfusion. 【Conclusion】 The 0.7~0.8 hematocrit concentrated washed RBCs prepared by the new approach is consistent with the special blood requirements during fetal anaemia transfusion, meets the clinical treatment standards, and can be applied in clinical.