To remove monoclonal anti-CD38 interference from compatibility testing: A simple method
10.13303/j.cjbt.issn.1004-549x.2021.09.010
- VernacularTitle:去除CD38单抗对输血相容性检测干扰的简便方法的建立
- Author:
Jie SONG
1
;
Yongkui KONG
1
;
Shuya WANG
1
;
Xianping LV
1
Author Information
1. Department of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Publication Type:Journal Article
- Keywords:
monoclonal anti-CD38;
antibody screening;
cross-matching;
alloantibody
- From:
Chinese Journal of Blood Transfusion
2021;34(9):974-977
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore a simple method to remove the interference of monoclonal anti-CD38 from compatibility testing and evaluate its effectiveness and safety, in order to develop a reasonable clinical transfusion strategy. 【Methods】 Blood phenotype detection, direct antiglobulin testing(DAT) and antibody screening were carried out by standard methods. Antibody screening and cross-matching of serums after monoclonal anti-CD38 treatment were performed by anti-human globulin card with 0.2 mol/L or 0.04 mol/L dithiothreitol(DTT) treated red blood cells. 【Results】 The results showed that 0.04 mol/L DTT treated directly in the anti-human globulin card for 15 min can completely remove the interference of monoclonal anti-CD38 in antibody screening and cross-matching without compromising of the yielding of anti-K, anti-LW, anti-JMH and anti-Lub alloantibodies. However, the titer of IgM antibodies may decrease in different degrees, and antibody screening and cross-matching with saline methods are required to avoid the missed detection of IgM alloantibodies. All 12 patients had no acute or delayed haemolytic transfusion reactions and their routine blood tests showed that the red blood cells transfusion were effective. 【Conclusion】 Based on antibody screening and cross-matching plus saline method, the method of 0.04 mol/L DTT, treated directly in the anti-human globulin card, is safe, effective and simple, which can detect most alloantibodies.
