Phenotype and RHD gene polymorphism of 64 Rh negative individuals
10.13303/j.cjbt.issn.1004-549x.2022.03.011
- VernacularTitle:Rh阴性个体表型及RHD基因多态性研究
- Author:
Miao HE
1
;
Na MA
1
;
Weiqun DONG
2
;
Sui WU
2
Author Information
1. Department of Blood Transfusion, Yunnan Provincial Cancer Hospital, Kunming 650118, China
2. Department of Blood Transfusion, The First Affiliated Hospital of Kunming Medical University
- Publication Type:Journal Article
- Keywords:
RhD negative;
RHD gene;
genotyping;
Rh phenotype
- From:
Chinese Journal of Blood Transfusion
2022;35(3):275-278
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To collect blood samples of 64 RhD negative patients in our hospital for RHD genotyping and phenotype analysis (RhC/c/E/e), and analyze the distribution characteristics of different RHD genotypes. 【Methods】 The RHD gene of RhD negative patients was genotyped by fluorescence quantitative polymerase chain reaction (PCR) method. The Rh phenotype was identified by IgM anti-e, anti-c, anti-C and anti-E, respectively. 【Results】 Forty-two cases of RHD deletion were detected, dominated by ccee phenotype (88.1%); 9 of RHD1227A cases, dominated by Ccee phenotype(77.8%); 8 of RHD-CE(3-9)-D2 cases, dominated by Ccee phenotype (75%); 1 of RHD-CE(3-10)-D2 case with Ccee phenotype, 1 of RHD*711delC case; 1 of RHAG site invalid type were detected. The typing results could not be determined in 2 cases by PCR method. 【Conclusion】 RhD negative patients showed diversity in RHD genotype, dominated by RHD deletion, followed by RHD1227A, RHD-CE(2-9)-D2, RHD-CE(3-10)-D2, RHD*711delC and RHAG site deletions.
