Collection of autologous peripheral hematopoietic stem cells in patients with sequential double transplantation of solid tumors and effect analysis
10.13303/j.cjbt.issn.1004-549x.2022.03.009
- VernacularTitle:实体瘤序贯双次移植患者自体造血干细胞采集方法及效果分析
- Author:
Ying ZHANG
1
;
Yushuang LIU
1
;
Qianxia ZHUANG
1
;
Nanhai WU
1
;
Zhouyang LIU
1
;
Hongyan LIU
1
;
Qi LIU
1
;
Zikuan GUO
1
;
Yuan SUN
1
Author Information
1. Department of Hematology/Oncology, Beijing Jingdu Children's Hospital, Beijing 102208, China
- Publication Type:Journal Article
- Keywords:
solid tumor;
double autologous hematopoietic stem cell transplantation;
cell collection;
low weight pediatric patients
- From:
Chinese Journal of Blood Transfusion
2022;35(3):269-271
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the methods and safety of autologous peripheral hematopoietic stem cells collection in patients with sequential double transplantation of solid tumors and conduct efficacy analysis. 【Methods】 Peripheral blood stem cells were collected from 27 patients with solid tumors after routine mobilization of rhG-CSF and rhGM-CSF.A specific program was made for the patients.The condition and cooperation degree of children were comprehensively evaluated before cell collection, and a femoral venous catheterization was inserted to ensure the cells collected smoothly.A mononuclear cell collection(MNC) program was selected, and machine parameters were set based on the patient's low body weight.The number of mononuclear cell (MNC) and the CD34+ cell was detected by flow cytometry for retrospective analysis. 【Results】 A total of 73 cell collections were performed in 27 patients, and the number of mononuclear cells and CD34+ cells was 12.586(10.22~19.586)×108/kg and 13.575(7.275~23.825)×106/kg, respectively, which can meet the requirement of sequential double transplantation. No intoxication of citrate and other serious adverse reactions occurred, and the follow-up was generally in good condition. 【Conclusion】 The method is effective and safe for pediatric patients, even for pediatric patients with low weight. Sufficient stem cells can be collected for patients with solid tumors by this method to meet the requirement of sequential double transplantation.
