Detection and analysis of neutralizing antibodies against SARS-CoV-2 in convalescent plasma donors
10.13303/j.cjbt.issn.1004-549x.2022.03.003
- VernacularTitle:新冠康复者恢复期血浆抗SARS-CoV-2中和抗体检测分析
- Author:
Yuan ZHANG
1
;
Lirong WANG
2
;
Jin DING
1
;
Jing LAN
1
;
Peng PENG
1
;
Na LIU
1
;
Junzhe ZHANG
1
;
Hua XU
1
Author Information
1. Shaanxi Blood Center, Xian 710061, China
2. School of Public Health, Xi’an Jiaotong University Health Science Center
- Publication Type:Journal Article
- Keywords:
COVID-19;
SARS-CoV-2;
convalescent plasma;
CMIA;
neutralizing antibody
- From:
Chinese Journal of Blood Transfusion
2022;35(3):245-249
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To detect the anti-SARS-CoV-2 neutralizing antibody levels in convalescent plasma (CP) and to evaluate whether it has specific anti-SARS-CoV-2 S antigen effect, so as to provide laboratory data support for clinical use of CP. 【Methods】 Nine CP donors who have recovered from COVID-19 were studied, and 4 volunteers who completed the vaccination and 3 asymptomatic infected blood donors were compared. Anti-SARS-CoV-2 antibodies including total antibody, IgM and IgG were measured by chemiluminescence microparticle immunoassays (CMIA) test in three groups. The VSV pseudovirus-based neutralization assay for evaluating neutralizing antibodies against SARS-CoV-2 was carried out in all samples. 【Results】 All samples were tested positive by the total antibody and IgG CMIA in COVID-19 CP donors and recipients of inactivated COVID-19 vaccine. High titers of IgG were observed in CP donors and vaccine recipients compared with asymptomatic blood donors. All vaccine recipients and 8 of 9 CP donors tested positive by SARS-CoV-2 pseudovirus-based neutralization test, whereas all asymptomatic blood donors tested negative. 【Conclusion】 The levels and characteristics of neutralizing antibodies among COVID-19 CP donors, vaccine recipients and asymptomatic blood donors were different. When unable to implement the pseudovirus assay to measure neutralizing antibodies, the detection of total antibody can be considered instead.
