HIV Ag-Abdetection by ELISA: a retrospective analysis
10.13303/j.cjbt.issn.1004-549x.2022.02.023
- VernacularTitle:ELISA检测HIV抗原抗体的回顾性分析
- Author:
Liangxue WEN
1
;
Ling WANG
1
;
Li SU
1
;
Mingfen WANG
1
Author Information
1. Zunyi Blood Center, Zunyi 563000, China
- Publication Type:Journal Article
- Keywords:
laboratory quality monitoring indicator;
external quality assessment;
retrospective analysis
- From:
Chinese Journal of Blood Transfusion
2022;35(2):216-218
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To conduct a retrospective analysis of HIV Ag-Ab detection by ELISA in our blood center, so as to provide reference for continuous improvement. 【Methods】 The reactive rate of HIV by each reagent, reactive rate of both reagents, re-test rate, concordance rate of initial-repeat test, and reagent utilization rate were counted, and the external quality assessment results were analyzed by PT score and z-ratio. 【Results】 The total reactive rate of HIV was 0.15%. The reactive rate by both reagents was 0.02%. The re-test rates, reactive rates, concordance rates and reagent utilization rates of the two reagents were 0.09% vs 0.08%, 0.07% vs 0.06%, 75.86% vs 78.21%, and 114.54% vs 113.92%, respectively. PT score was 100%, and z-ratio of 7 negative samples and 1 positive sample was less than 2, and of 2 positive samples was more than 3. 【Conclusion】 The laboratory quality monitoring indicators and external quality assessment can effectively monitor the operation of blood testing laboratory.
